biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:657-662, 2009 | DOI: 10.1007/s10535-009-0119-7

QTLs mapping of physiological traits related to salt tolerance in young rice seedlings

H. Sabouri1,*, A. M. Rezai2, A. Moumeni3, A. Kavousi3, M. Katouzi3, A. Sabouri4
1 Department of Plant Production, Gonbad High Education Center, Gorgan University of Agriculture Science and Natural Resource, Gonbad, Iran
2 Faculty of Agriculture Sciences, Isfahan University of Technology, Isfahan, Iran
3 Researchers in Plant Breeding, Rice Research Institute of Iran, Rasht, Iran
4 Faculty of Agriculture Sciences, University of Guilan, Rasht, Iran

Oryza sativa L. F2 population and F2:3 derived from a cross between salt tolerance cv. Tarommahali and salt sensitive cv. Khazar were used in this study. A linkage map based on F2 population was constructed (74 SSR markers on 192 individuals), which covered a total of 1231.50 cM with an average two locus interval of 19.83 cM. Two QTLs related to Na+/K+ ratio were found on chromosome 3 and 6. qDM-3 and qDM-8 (for dry mass of shoot) are major QTLs with very large effects explained 20.90 and 17.72 % of the total phenotypic variance, respectively. Major locus for DM (qDM-3) was bracketed by RM1022 - RM6283 spread over 13.6 cM on chromosome 3. Major part of the variability for standard tolerance ranking (STR) was explained by the qSTR-6 flanked by RM3727 - RM340 on chromosome 6, which exhibited phenotypic variance of 17.25 % and peak likelihood ratio (LR) of 17.51. The length of this QTL is 8.8 cM and identification of any tightly linked markers in this region will serve as a candidate gene for fine-mapping. qSTR-3 overlapped with qNA-3 and qNAK-3. The qSTR-3 may contain a new major gene for salt stress tolerance at seedling stage in rice. Major QTLs identified in this paper, after fine-mapping, could be used for marker assisted selection.

Keywords: marker-assisted selection; Oryza sativa; SSR
Subjects: Oryza sativa; potassium; quantitative trait loci (QTL); rice; salinity; sodium

Received: January 31, 2008; Accepted: June 3, 2008; Published: December 1, 2009  Show citation

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Sabouri, H., Rezai, A.M., Moumeni, A., Kavousi, A., Katouzi, M., & Sabouri, A. (2009). QTLs mapping of physiological traits related to salt tolerance in young rice seedlings. Biologia plantarum, 53(4), 657-662. doi: 10.1007/s10535-009-0119-7
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