Raw data, assembly and annotation results for: “Ultra-deep sequencing enables high-fidelity recovery of biodiversity for bulk arthropod samples without PCR amplification”

The following data comes from the study “Ultra-deep sequencing enables high-fidelity recovery of biodiversity for bulk arthropod samples without PCR amplification”, which is the first systematic meta-barcoding study sequencing the total DNA from insect communities independent of PCR amplification. Following mitochondrial enrichment using differential centrifugation, a preliminary sample and a formal sample are achieved respectively with 2.2G and 13.2G from the Illumina HiSeq 2000 platform using 100 bp paired-end (PE) sequencing, following manufacturer’s instructions. The data was assembled using SOAPdenovo2 and annotated using the MT_annotation_BGI pipeline (tools and pipelines available here: doi:10.5524/100046). Further details are available in the published paper and the metadata provided in the MIMS formatted table.

Read the peer-reviewed publication(s):

• Zhou, X., Li, Y., Liu, S., Yang, Q., Su, X., Zhou, L., Tang, M., Fu, R., Li, J., & Huang, Q. (2013). Ultra-deep sequencing enables high-fidelity recovery of biodiversity for bulk arthropod samples without PCR amplification. GigaScience, 2(1). https://doi.org/10.1186/2047-217x-2-4 (PubMed:23587339)

Related datasets:

doi:10.5524/100045 IsSupplementedBy doi:10.5524/100046

Accessions (data included in GigaDB):

SRA: SRA067357