Micro-computed tomography scans and virtual histological slides of a recently described land planarian species of Obama otavioi (Platyhelminthes).
Dataset type: Imaging
Data released on March 01, 2016
Carbayo F; Lenihan JW (2016): Micro-computed tomography scans and virtual histological slides of a recently described land planarian species of Obama otavioi (Platyhelminthes). GigaScience Database. https://doi.org/10.5524/100190
We investigated whether images obtained through X-ray micro-computed tomography (μCT), can be used in conjunction with traditional methods for morphological studies of soft-bodied land planarians. Among its virtues, μCT is non-invasive and provides true to scale three-dimensional imagery at high resolution. We compared these μCT-based images with those obtained from light microphotography of histological sections, most of which were also digitizseddigitized at high magnification. The specimens studied were collected in 2012. Subsequent μCT-based images of the stained body of a paratype show nearly all morphological features provided by traditional histology, with the exception of structures particularly minute, 5 μm, such as the sensory pits and single muscle fibers, which are best visible on traditional histological sections. Since the technique is non-destructive, the scanned specimen is preserved without damage. The raw and derivative µCT data and virtual histological sections can be found here.
Additional details
Read the peer-reviewed publication(s):
- Carbayo, F., Francoy, T. M., & Giribet, G. (2016). Non‐destructive imaging to describe a new species of Obama land planarian (Platyhelminthes, Tricladida). Zoologica Scripta, 45(5), 566–578. Portico. https://doi.org/10.1111/zsc.12175
- Carbayo, F., & Lenihan, J. W. (2016). Micro-computed tomography scan and virtual histological slide data for the land planarian Obama otavioi (Platyhelminthes). GigaScience, 5(1). https://doi.org/10.1186/s13742-016-0119-4
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Table SettingsSample ID | Common Name | Scientific Name | Sample Attributes | Taxonomic ID | Genbank Name |
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MZUSP PL 1574 | Obama sp. | Obama sp. | Sex:hermaphrodite Collection date:22 / Sept / 2012. Environment (feature):forest [ENVO:00000111] ... |
1336495 | |
MZUSP PL 1573 | Obama sp. | Obama sp. | Sex:hermaphrodite Broad-scale environmental context:tropical moist b... Collection date:22 / Sept / 2012. ... |
1336495 | |
MCZ 59100 | Obama sp. | Obama sp. | Sex:hermaphrodite Environment (feature):forest [ENVO:00000111] Species-a:Obama otavioi ... |
1336495 |
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Table SettingsFile Name | Description | Sample ID | Data Type | File Format | Size | Release Date | File Attributes | Download |
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Readme | TEXT | 3.55 kB | 2016-02-02 | MD5 checksum: 2d7f67b1be68231753b1b8b9f63eef99 |
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ΜCT-based projection images of entire specimen | Mixed archive | TAR | 58.83 GB | 2016-02-02 | MD5 checksum: f06057ea39dc032b76b7bd400cb7d7b4 |
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MCT-based volumetric image stacks of entire body | Mixed archive | TAR | 1.10 GB | 2016-02-02 | MD5 checksum: 5bd67f73d0878f3610dba416157038da |
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ΜCT-based projection images of anterior end of the body | Mixed archive | TAR | 67.52 GB | 2016-02-02 | MD5 checksum: 8b9ad8d8c90f6be4c6d351c2b5ccbeb6 |
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MCT-based volumetric image stacks of anterior end of the body | Mixed archive | TAR | 1.04 GB | 2016-02-02 | MD5 checksum: 47722e2393b9b20862ce91e9980ae1da |
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ΜCT-based projection images of pharynx | Mixed archive | TAR | 22.91 GB | 2016-02-02 | MD5 checksum: d388cabeff877f48fd815b65d9ea13a4 |
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pharynx | Mixed archive | TAR | 1.41 GB | 2016-02-02 | MD5 checksum: 89b2c00eedd14cece86b0a668d54500f |
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ΜCT-based projection images of copulatory apparatus | Mixed archive | TAR | 22.92 GB | 2016-02-02 | MD5 checksum: a718e0fe59edc647249e7de6225510a8 |
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MCT-based volumetric image stacks of copulatory apparatus | Mixed archive | TAR | 1.12 GB | 2016-02-02 | MD5 checksum: 298021046d5a843f32e51c196714ca2f |
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virtual histological slides of anterior end | Mixed archive | TAR | 917.89 MB | 2016-02-02 | MD5 checksum: ea07df0a447386738c2aa30db877c13d |
Funding body | Awardee | Award ID | Comments |
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São Paulo Research Foundation | Proc. 2014/13661–8 | Fernado Carbayo |
Date | Action |
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February 29, 2016 | Dataset publish |
March 7, 2016 | Manuscript Link added : 10.1111/zsc.12175 |
March 17, 2016 | Manuscript Link added : 10.1186/s13742-016-0119-4 |