biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:85-91, 2005 | DOI: 10.1007/s10535-005-5091-2

Differences in antioxidant activity in response to salinity stress in tolerant and susceptible wheat genotypes

R. K. Sairam1,*, G. C. Srivastava1, S. Agarwal1, R. C. Meena1
1 Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, India

Effects of long-term sodium chloride salinity (100 and 200 mM NaCl; ECe = 6.85 and 12.3 dS m-1) were studied in tolerant (Kharchia 65, KRL 19) and susceptible (HD 2009, HD 2687) wheat genotypes. NaCl decreased relative water content (RWC), chlorophyll content (Chl), membrane stability index (MSI) and ascorbic acid (AA) content, and increased the contents of hydrogen peroxide, thiobarbituric acid reactive substances (TBARS), and activities of superoxide dismutase (SOD), ascorbate peroxidase (APOX) and glutathione reductase (GR). Kharchia 65 showed lowest decline in RWC, Chl, MSI and AA content, lowest increase in H2O2 and TBARS contents and higher increase in SOD and its isozymes, APOX and GR, while HD2687 showed the highest decrease in AA content, highest increase in H2O2 and TBARS contents and smallest increase in activities of antioxidant enzymes. KRL 19 and HD 2009 showed intermediate response both in terms of oxidative stress and antioxidant activity.

Keywords: ascorbate peroxidase; ascorbic acid; chlorophyll; glutathione reductase; lipid peroxidation; superoxide dismutase; Triticum aestivum
Subjects: antioxidants, enzymes; antioxidants, salt stress; ascorbate peroxidase; chlorophylls, salt stress; cultivar and genotype differences, salt stress; glutathione reductase; lipid peroxidation; membrane stability index (MSI); oxidative stress, antioxidants; reactive oxygen species (ROS); relative water content; salt stress, antioxidant enzymes; salt stress, membrane stability index, RWC; superoxide dismutase; thiobarbituric acid; Triticum aestivum; wheat, contrasting genotypes

Received: November 14, 2003; Accepted: May 24, 2004; Published: March 1, 2005  Show citation

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Sairam, R.K., Srivastava, G.C., Agarwal, S., & Meena, R.C. (2005). Differences in antioxidant activity in response to salinity stress in tolerant and susceptible wheat genotypes. Biologia plantarum, 49(1), 85-91. doi: 10.1007/s10535-005-5091-2
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