biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:110-115, 2007 | DOI: 10.1007/s10535-007-0022-z

Salinity induced changes in proline and betaine contents and synthesis in two aquatic macrophytes differing in salt tolerance

S. B. Tripathi1, K. Gurumurthi2, A. K. Panigrahi3, B. P. Shaw4,*
1 TERI, Habitat Place, New Delhi, India
2 IFGTB, Coimbatore, India
3 Dept. of Botany, Berhampur University, Berhampur, India
4 Institute of Life Science, Nalco Square, Bhubaneswar, India

The present work investigated the effect of NaCl and seawater salinity (SWS) on accumulation of proline and glycinebetaine (betaine) and expression of two key enzymes of their biosynthetic pathway, betainealdehyde dehydrogenase (BADH) and Δ1-pyrroline-5-carboxylate synthetase (P5CS) in two aquatic macrophytes, salt-tolerant Najas gramenia and moderately-salt-tolerant Najas indica. N. gramenia did not show any accumulation of betaine or expression of BADH in response to NaCl or SWS treatments. The content of proline increased significantly in response to the salt treatments. High expression of P5CS was also observed, however, the transcript abundance did not correlate with the amount of proline. N. indica, exhibited significantly greater accumulation of proline in response to SWS, which contained Ca2+ in addition to NaCl, than in response to NaCl alone.

Keywords: betaine aldehyde dehydrogenase; NaCl; Najas gramenia; Najas indica; Δ1-pyrroline-5-carboxylate synthetase; seawater salinity
Subjects: betaine aldehyde dehydrogenase; Najas gramenia; Najas indica; Northern blot analyses; osmotic adjustment; pyrroline-5-carboxylate reductase; proline; salinity, salt stress; Southern blot analysis; synthetase

Received: February 14, 2005; Accepted: January 15, 2006; Published: March 1, 2007  Show citation

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Tripathi, S.B., Gurumurthi, K., Panigrahi, A.K., & Shaw, B.P. (2007). Salinity induced changes in proline and betaine contents and synthesis in two aquatic macrophytes differing in salt tolerance. Biologia plantarum, 51(1), 110-115. doi: 10.1007/s10535-007-0022-z
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