biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:535-538, 2010 | DOI: 10.1007/s10535-010-0094-z

Genetic diversity in Passiflora species determined by morphological and molecular characteristics

A. J. C. Viana1, M. M. Souza1,*, I. S. Araújo1, R. X. Corrêa1, D. Ahnert1
1 Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, Brasil

Morphological and molecular characteristics were studied in six wild species of Passiflora. There were statistically significant differences among these six species for all characteristics studied. Intra-specific variability was observed for number of flowers, number of fruits, number of seeds, fruit length, fruit width and leaf area. Cluster analysis using morphological data showed three groups: 1) P. palmeri var. sublanceolata, P. morifolia and P. foetida var. foetida, 2) P. coriacea and P. micropetala, and 3) P. suberosa. The dendrogram constructed using randomly amplified polymorphic DNA (RAPD) data showed six different groups for each species. The genetic distances among the 24 accessions ranged from 0.05 (between P. morifolia accessions P1 and P3) to 0.95 (P. coriacea accession 31 and P. palmeri var. sublanceolata accession 49). The species showed high morphological and molecular inter- and intraspecific variability.

Keywords: passion fruit; polymerase chain reaction; RAPD markers
Subjects: genetic diversity; Passiflora spp.; polymerase chain reaction (PCR); random amplified polymorphic DNA (RAPD); UPGMA dengrogram

Received: September 19, 2008; Accepted: June 2, 2009; Published: September 1, 2010  Show citation

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Viana, A.J.C., Souza, M.M., Araújo, I.S., Corrêa, R.X., & Ahnert, D. (2010). Genetic diversity in Passiflora species determined by morphological and molecular characteristics. Biologia plantarum, 54(3), 535-538. doi: 10.1007/s10535-010-0094-z
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