biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 55:105-111, 2011 | DOI: 10.1007/s10535-011-0014-x

Combined drought and heat stress in wheat: changes in some heat shock proteins

B. Grigorova1,*, I. Vaseva1, K. Demirevska1, U. Feller2
1 Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
2 Institute of Plant Sciences and Oeschger Centre for Climate Change Research, University of Bern, Bern, Switzerland

The influence of combined and individually applied drought and heat stress was studied in two wheat (Triticum aestivum L.) cultivars: resistant cv. Katya and susceptible cv. Sadovo. Relative water content decreased and electrolyte leakage increased due to individual and combined application of both stresses. Initial heat shock protein profile has been outlined via SDS electrophoresis of leaf extracts. The results obtained were confirmed by immunoblotting with anti-HSP70 monoclonal antibodies, anti-HSP110 polyclonal antibodies and anti-α β-crystalline polyclonal antibodies. The effect of simultaneously applied water stress and heat shock resembled the alterations in protein expression provoked only by water stress and differed significantly from the changes occurring after the individual application of heat stress.

Keywords: electrolyte leakage; immunoblotting; relative water content; Triticum aestivum

Received: June 15, 2009; Accepted: January 17, 2010; Published: March 1, 2011  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Grigorova, B., Vaseva, I., Demirevska, K., & Feller, U. (2011). Combined drought and heat stress in wheat: changes in some heat shock proteins. Biologia plantarum, 55(1), 105-111. doi: 10.1007/s10535-011-0014-x
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Barrs, H.D., Weatherley, P.E.: A re-examination of the relative turgidity technique for estimating water deficit in leaves. - Aust. J. biol. Sci. 15: 413-428, 1962. Go to original source...
    2. Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of proteins using the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    3. Caeiro, A.S., Ramos, P.C., Teixeira, A.R., Ferreira, R.B.: The ubiquitin/proteasome pathway from Lemna minor subjected to heat shock. - Biol. Plant. 52: 695-702, 2008. Go to original source...
    4. Chaves, M.M., Oliveira, M.M.: Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. - J. exp. Bot. 55: 2365-2384, 2004. Go to original source...
    5. Demirevska, K., Simova-Stoilova, L., Vassileva, V., Feller, U.: Rubisco and some chaperone protein responses to water stress and rewatering at early seedling growth of drought sensitive and tolerant wheat varieties. - Plant Growth Regul. 56: 97-106, 2008. Go to original source...
    6. Ferguson, B.: The plant response: stress in the daily environment. - J. Zhejiang Univ. Sci. 5: 129-132, 2004. Go to original source...
    7. Geneva, M., Zehirov, G., Djonova, E., Kaloyanova, N., Georgiev, G., Strancheva, I.: The effect of inoculation of pea plants with mycorrhizal fungi and Rhizobium on nitrogen and phosphorus assimilation. - Plant Soil Environ. 52: 435-440, 2006. Go to original source...
    8. Gulli, M., Corradi, M., Rampino, P., Marmiroli, N., Perrotta, C.: Four members of the HSP101 gene family are differently regulated in Triticum durum Desf. - FEBS Lett. 581: 4841-4849, 2007. Go to original source...
    9. Jiang, Y., Huang, B.: Protein alterations in tall fescue in response to drought stress and abscisic acid. - Crop Sci. 42: 202-207, 2002. Go to original source...
    10. Kalapos, T., Van den Boogaard, R., Lambers, H.: Effect of soil drying on growth, biomass allocation and leaf gas exchange of two annual grass species. - Plant Soil 185: 137-149, 1996. Go to original source...
    11. Kotak, S., Larkindale, J., Lee, U., Pascal von Koskull-Doring, Vierling, E., Scharf, K.: Complexity of the heat stress response in plants. - Plant Biol. 10: 310-316, 2007. Go to original source...
    12. Kregel, K.: Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance. - J. appl. Physiol. 92: 2177-2186, 2002. Go to original source...
    13. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. - Nature 277: 680-685, 1970. Go to original source...
    14. Lee, G., Vierling, E.: A small heat shock protein cooperates with heat shock protein 70 system to reactivate a heat-denaturated protein. - Plant Physiol. 122: 189-197, 2000. Go to original source...
    15. Lee, U., Wie, C., Escobar, M., Williams, B., Hong, S., Vierling, E.: Genetic analysis reveals domain interactions of Arabidopsis HSP100/ClpB and cooperation with the small heat shock protein chaperone system. - Plant Cell 17: 559-571, 2005. Go to original source...
    16. Lin, Q., Wang, Y.M., Nose, A., Hong, H.T.K., Agarie, S.: Effect of high night temperature on lipid and protein compositions in tonoplasts isolated from Ananas comosus and Kalanchoë pinnata leaves. - Biol. Plant. 52: 59-65, 2008. Go to original source...
    17. Mansfield, M., Key, J.: Synthesis of the low molecular weight heat shock proteins in plants. - Plant Physiol. 84: 1007-1017, 1987. Go to original source...
    18. Mayer, M., Bukau, B.: Hsp70 chaperones: cellular functions and molecular mechanism. - Cell Mol. Life Sci. 62: 670-684, 2005. Go to original source...
    19. Miernyk, J.A.: Protein folding in the plant cell. - Plant Physiol. 121: 695-703, 1999. Go to original source...
    20. Mitsuhashi, W., Feller, U.: Effects of light and external solutes on the catabolism of nuclear-encoded stromal proteins in intact chloroplasts isolated from pea leaves. - Plant Physiol. 100: 2100-2105, 1992. Go to original source...
    21. Mittler, R.: Abiotic stress, the field environment and stress combination. - Trends Plant Sci. 11: 15-19, 2006. Go to original source...
    22. Mittler, R., Merquiol, E., Hallak-Herr, E., Rachmilevitch, S., Kaplan, A., Cohen, M.: Living under a 'dormant' canopy: a molecular acclimation mechanism of the desert plant Retama raetam. - Plant J. 25: 407-416, 2001. Go to original source...
    23. Moffat, A.S.: Finding new ways to protect drought-stricken plants. - Science 296: 1226-1229, 2002. Go to original source...
    24. Nunes, M.E.S., Smith, G.R.: Electrolyte leakage assay capable of quantifying freezing resistance in rose clover. - Crop Sci. 43: 1349-1357, 2003. Go to original source...
    25. Oh, H., Easton, D., Murawski, M., Kaneko, Y., Subjeck, J.: The chaperoning activity of HSP110. - J. biol. Chem. 274: 15712-15718, 1999. Go to original source...
    26. Queitsch, C., Hong, S., Vierling, E., Lindquist, S.: Heat shock protein 101 plays a crucial role in thermotolerance in Arabidopsis. - Plant Cell 12: 479-492, 2000. Go to original source...
    27. Rizhsky, L., Liang, H., Mittler, R.: The combined effect of drought stress and heat shock on gene expression in tobacco. - Plant Physiol. 130: 1143-1151, 2002. Go to original source...
    28. Rizhsky, L., Liang, H., Shuman, J., Shulaev, V., Davletova, S., Mittler, R.: When defense pathways collide. The response of Arabidopsis to a combination of drought and heat stress. - Plant Physiol. 134: 1683-1696, 2004. Go to original source...
    29. Ritossa, F.: A new puffing pattern induced by temperature shock and DNP in Drosophila. - Experientia 18: 571-573, 1962. Go to original source...
    30. Santhoshkumar, P., Sharma, K.: Conserved F84 and P86 residues in αβ-crystallin are essential to effectively prevent the aggregation of substrate proteins. - Prot. Sci. 15: 2488-2498, 2006. Go to original source...
    31. Santos, M.G., Ribeiro, R.V., Machado, E.C., Pimentel, C.: Photosynthetic parameters and leaf water potential of five common bean genotypes under mild water deficit. - Biol. Plant. 53: 229-236, 2009. Go to original source...
    32. Schoffl, F., Prandl, R., Reindl, A.: Regulation of the heat-shock response. - Plant Physiol. 117: 1135-1141, 1998. Go to original source...
    33. Simova-Stoilova, L., Vassileva, V., Demirevska, K., Feller, U.: Proteolytic activity in wheat leaves during drought stress and recovery. - Gen. Appl. Plant Physiol. 31(Spec. Issue): 91-100, 2006.
    34. Smykal, P., Masin, J., Hardy, I., Konopasek, I., Zarsky, V.: Chaperone activity of tobacco HSP18, a small heat-shock protein, is inhibited by ATP. - Plant J. 23: 703-713, 2000. Go to original source...
    35. Sumesh, K.V., Sharma-Natu, P., Ghildiyal, M.C.: Starch synthase activity and heat shock protein in relation to thermal tolerance of developing wheat grains. - Biol. Plant. 52: 749-753, 2008. Go to original source...
    36. Vierling, E.: The roles of heat shock proteins in plants. - Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 579-620, 1991. Go to original source...
    37. Wang, W., Vinocur, B., Shoseyov, O., Altman, A.: Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. - Trends Plant Sci. 9: 244-252, 2004. Go to original source...
    38. Waters, E., Lee, G., Vierling, E.: Evolution, structure and function of the small heat shock proteins in plants. - J. exp. Bot. 47: 325-338, 1996. Go to original source...

    Return to the content