biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:37-42, 2012 | DOI: 10.1007/s10535-012-0013-6

Constitutive expression of Arabidopsis DREB1B in transgenic potato enhances drought and freezing tolerance

S. Movahedi1, B. E. Sayed Tabatabaei1,*, H. Alizade2, C. Ghobadi1, A. Yamchi3, G. Khaksar1
1 Department of Agricultural Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran
2 Department of Agricultural Biotechnology, College of Agriculture, University of Tehran, Tehran, Iran
3 Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran

DNA cassette consisting of an Arabidopsis dehydration-responsive element binding factor 1 (DREB1B) cDNA, driven by a cauliflower mosaic virus 35S promoter, was introduced into potato plants (Solanum tuberosum L.) through Agrobacterium tumefaciens-mediated gene transfer. The presence and expression of the gene in transgenic plants were confirmed by the PCR and RT-PCR techniques, respectively. Northern hybridization using a DREB1B cDNA probe revealed high levels of DREB1B expression among the most transgenic lines. Overexpression of DREB1B imparted a significant freezing and drought tolerance gain in the transgenic potato lines. In comparison with the wild-type plants, the transgenic potatoes contained higher proline content under drought and freezing conditions, and maintained their relative water content higher under water stress. The enhancement of tolerance in transgenic potato highlights the presence of genes responding to the transcription factor DREB1B in this plant.

Keywords: abiotic stress; dehydration-responsive element; overexpression; Solanum tuberosum; transcription factor
Subjects: abiotic stress; dehydration-responsive element; transcription factor; gene overexpression; water content; water stress; temperature - low; proline; Northern blot

Received: August 15, 2010; Accepted: December 8, 2010; Published: March 1, 2012  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Movahedi, S., Sayed Tabatabaei, B.E., Alizade, H., Ghobadi, C., Yamchi, A., & Khaksar, G. (2012). Constitutive expression of Arabidopsis DREB1B in transgenic potato enhances drought and freezing tolerance. Biologia plantarum, 56(1), 37-42. doi: 10.1007/s10535-012-0013-6
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Achard, P., Gong, F., Cheminant, S., Alioua, M., Hedden, P., Genschik, P.: The cold-inducible CBF1 factor-dependent signaling pathway modulates the accumulation of the growth-repressing DELLA proteins via its effect on gibberellin metabolism. - Plant Cell 20: 2117-2129, 2008. Go to original source...
    2. Agarwal, P.K., Agarwal, P., Reddy, M.K., Sopory, S.K.: Role of DREB transcription factors in abiotic and biotic stress tolerance in plants. - Plant Cell Rep. 25: 1263-1274, 2006. Go to original source...
    3. Angell, S.M., Baulcombe, D.C.: Consistent of gene silencing in transgenic plants expressing a replicating potato virus X RNA. - EMBO J. 16: 3675-3684, 1997. Go to original source...
    4. Banerjee, A.K., Prat, S., Hannapel, D.: Efficient production of transgenic potato (S. tuberosum L. ssp. andigena) plants via Agrobacterium tumefaciens-mediated transformation. - Plant Sci. 170: 732-738, 2006. Go to original source...
    5. Bates, L.S.: Rapid determination of free proline for water stress studies. - Plant Soil 39: 205-207, 1973. Go to original source...
    6. Behnam, B., Kikuchi, A., Celebi-Toprak, F., Kasuga, M., Yamaguchi-Shinozaki, K., Watanabe, K.: Arabidopsis rd29A:DREB1A enhances freezing tolerance in transgenic potato. - Plant Cell Rep. 26: 1275-1282, 2007. Go to original source...
    7. Bhatnagar-Mathur, P., Devi, M.J., Reddy, D.S., Lavanya, M., Vadez, V., Serraj, R., Yamaguchi-Shinozaki, K., Sharma, K.K.: Stress-inducible expression of AtDREB1A in transgenic peanut (Arachis hypogaea L.) increases transpiration efficiency under water-limiting conditions. - Plant Cell Rep. 26: 2071-2082, 2007. Go to original source...
    8. Celebi-Toprak, F., Behnam, B., Serrano, G., Kasuga, M., Yamaguchi-Shinozaki, K., Naka, H., Watanabe, J.A., Yamanaka, S., Watanabe, K.N.: Tolerance to salt stress in transgenic tetrasomic tetraploid potato, Solanum tuberosum cv. Desiree appears to be induced by DREB1A gene and rd29A promoter of Arabidopsis thaliana. - Breed Sci. 55: 311-320, 2005. Go to original source...
    9. Gilmour, S.J., Fowler, S.G. Thomashow, M.F.: Arabidopsis transcription activators CBF1, CBF2 and CBF3 have matching functional activities. - Plant mol. Biol. 54: 767-781, 2004. Go to original source...
    10. Gilmour, S.J., Sebolt, A.M., Salazar, M.P., Everard, J.D. Thomashow, M.F.: Overexpression of the Arabidopsis CBF3 transcriptional activator mimics multiple biochemical changes associated with cold acclimation. - Plant Physiol. 124: 1854-1865, 2000. Go to original source...
    11. Hoekstra, F.A., Golovina, E.A. Buitink, J.: Mechanisms of plant desiccation tolerance. - Trends Plant Sci. 6: 431-438, 2001. Go to original source...
    12. Hsieh, T.H., Lee, J.T., Charng, Y.Y., Chan, M.T.: Tomato plants ectopically expression Arabidopsis CBF1 show enhanced resistance to water deficit stress. - Plant Physiol. 130: 618-626, 2002a. Go to original source...
    13. Hsieh, T.H., Lee, J.T., Yang, P.T., Chiu, L.H., Charng, Y.Y., Wang, Y.C. Chan, M.T.: Heterology expression of the Arabidopsis C-repeat/dehydration response element binding factor 1 gene confers elevate tolerance to chilling and oxidative stress in transgenic tomato. - Plant Physiol. 129: 1086-1094, 2002b. Go to original source...
    14. Hu, X.J., Zhang, Z.B., Xu, P., Fu, Z.Y., Hu, S.B., Song, W.Y.: Multifunctional genes: the cross-talk among the regulation network of abiotic stress responses. - Biol. Plant. 54: 213-223, 2010. Go to original source...
    15. Jaglo, K.R., Kleff, S., Amundsen, K.L., Zhang, X., Haake, V., Zhang, J.Z., Deits, T., Thomashow, M.F.: Components of Arabidopsis C-repeat/dehydration responsive element binding factor cold-responsive pathways are conserved in Brassica napus and other plant species. - Plant Physiol. 127: 910-917, 2001. Go to original source...
    16. James, A.V., Neibaur, I., Altpeter, F.: Stress inducible expression of the DREB1A transcription factor from xeric, Hordeum spontaneum L. in turf and forage grass (Paspalum notatum Flugge) enhances abiotic stress tolerance. - Transgenic Res. 17: 93-104, 2008. Go to original source...
    17. Jefferson, R.A., Kavanagh, T.A., Bevan, M.W.: GUS fusions; beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. - EMBO J. 6: 3901-3907, 1987. Go to original source...
    18. Jeong, M.J., Park, S.C., Byun, M.O.: Improvement of salt tolerance in transgenic potato plants by glyceraldehydes-3-phosphate dehydrogenase gene transfer. - Mol. Cells 12: 185-189, 2001.
    19. Jin, T., Chang, Q., Li, W., Yin, D., Li, Z., Wang, D., Liu, B., Liu, L.: Stress-inducible expression of GmDREB1 conferred salt tolerance in transgenic alfalfa. - Plant Cell Tissue Organ Cult. 100: 219-227, 2009. Go to original source...
    20. Kasuga, M., Miura, S., Shinozaki, K., Yamaguchi-Shinozaki, K.: A combination of the Arabidopsis DREB1A gene and stress-inducible rd29A promoter improved drought- and low-temperature stress tolerance in tobacco by gene transfer. - Plant Cell Physiol. 45: 346-350, 2004. Go to original source...
    21. Lee, S.C., Huh, K.W, An, K., An, G., Kim, S.R.: Ectopic expression of a cold-inducible transcription factor, CBF1/DREB1B, in transgenic rice (Oryza sativa L.). - Mol. Cells 18: 107-114, 2004.
    22. Nakashima, K., Yamaguchi-Shinozaki, K.: Regulons involved in osmotic stress-responsive and cold stress-responsive gene expression in plants. - Physiol. Plant. 126: 62-71, 2006. Go to original source...
    23. Pellegrineschi, A., Reynolds, M., Pacheco, M., Brito, R.M., Almeraya, R., Yamaguchi-Shinozaki, K., Hoisington, D.: Stress-induced expression in wheat of the Arabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions. - Genome 47: 493-500, 2004. Go to original source...
    24. Rensink, W., Hart, A., Liu, J., Ouyang, Sh., Zismann, V., Robin, Buell, C.: Analyzing the potato abiotic stress transcriptome using expressed sequence tags. - Genome 48: 598-605, 2005. Go to original source...
    25. Richards, R.A., Rebetzke, G.J., Condon, A.G., Herwaarden, A.F.: Breeding opportunities for increasing the efficiency of water use and crop yield in temperate cereals. - Crop Sci. 42: 111-121, 2002. Go to original source...
    26. Tong, Z., Hong, B., Yang, Y., Li, Q., Ma, N., Ma, C., Gao, J.: Overexpression of two chrysanthemum DgDREB1 group genes causing delayed flowering or dwarfism in Arabidopsis. - Plant mol. Biol. 71: 115-129, 2009. Go to original source...
    27. Umezawa, T., Fujita, M., Fujita, Y., Yamaguchi-Shinozaki, K., Shinozaki, K.: Engineering drought tolerance in plants: discovering and tailoring genes to unlock the future. - Curr. Opin. Biotechnol. 17: 113-122, 2006. Go to original source...
    28. Xiong, L., Schumaker, K.S., Zhu, J.K.: Cell signaling during cold, drought and salt stress. - Plant Cell. 14: 165-183, 2002. Go to original source...
    29. Yamaguchi-Shinozaki, K., Shinozaki, K.: A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. - Plant Cell 6: 251-264, 1994. Go to original source...
    30. Yang, S., Vanderbeld, B., Wan, J., Huang, Y.: Narrowing down the targets: towards successful genetic engineering of drought-tolerant crops. - Mol. Plant. 3: 469-490, 2010. Go to original source...
    31. Zhang, J.Z., Creelman, R.A., Zhu, J.K.: From laboratory to field using information from Arabidopsis to engineer salt, cold, and drought tolerance in crops. - Plant Physiol. 135: 615-621, 2004. Go to original source...

    Return to the content