biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:139-143, 2013 | DOI: 10.1007/s10535-012-0260-6

The effect of elicitors on oleanolic acid accumulation and expression of triterpenoid synthesis genes in Gentiana straminea

Z. -J. Zhao1, Y. -G. Song1, Y. -L. Liu1, M. Qiao1, X. -L. Zhai2, F. -N. Xiang1,*
1 The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, School of Life Sciences, Shandong University, Jinan, P.R. China
2 Research Institution of Agriculture of Qingdao City, Qingdao, P.R. China

Gentiana straminea is native to the Qinghai-Tibet plateau, where it is exposed to extremes of cold and strong UV-B radiation. Here we showed that low temperature, but not UV-B radiation, affected the accumulation of the triterpenoid oleanolic acid. Neither of these stresses altered the expression of known triterpenoid synthesis genes. However, the application of methyl jasmonate (MeJA), but not salicylic acid (SA), significantly enhanced the accumulation of oleanolic acid, and up-regulated the triterpenoid synthesis genes, especially the expression of βAS, the gene encoding β-amyrin.

Keywords: β-amyrin synthase; low temperature; methyl jasmonate; salicylic acid; UV-B radiation
Subjects: elicitors; oleanolic acid; triterpenoid synthesis; β-amyrin synthase; temperature low; methyl jasmonate; salicylic acid; UV-B radiation

Received: April 14, 2011; Accepted: March 28, 2012; Published: March 1, 2013  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Zhao, Z.-J., Song, Y.-G., Liu, Y.-L., Qiao, M., Zhai, X.-L., & Xiang, F.-N. (2013). The effect of elicitors on oleanolic acid accumulation and expression of triterpenoid synthesis genes in Gentiana straminea. Biologia plantarum, 57(1), 139-143. doi: 10.1007/s10535-012-0260-6
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Chen, Q.L., Shi, Z.Y., Tu, G.Z., Sun, W.J.: Studies on the chemical constituents in root of Gentiana macrophylla from Shaanxi. - China J. Chin. Mat. Med. 19: 1519-1522, 2005.
    2. Choi, D.W., Jung, J.D., Ha, Y.I., Park, H.W., In, D.S., Chung, H.J., Liu, J.R.: Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites. - Plant Cell Rep. 23: 557-566, 2005. Go to original source...
    3. Dolzhenko, Y., Bertea, C.M., Occhipinti, A., Bossi, S., Maffei, M.E.: UV-B modulates the interplay between terpenoidsand flavonoids in peppermint (Mentha ×piperita L.). - J. Photochem. Photobiol. B: Biol. 100: 67-75, 2010. Go to original source...
    4. D'Onofrio, C., Cox, A., Davies, C., Boss, P.K.: Induction of secondary metabolism in grape cell cultures by jasmonates. - Funct. Plant Biol. 36: 323-338, 2009. Go to original source...
    5. Ebel, J.: Phytoalexin synthesis: the biochemical analysis of the induction process. - Annu. Rev. Phytopathol. 24: 235-264, 1986. Go to original source...
    6. Kim, O.T., Bang, K.H., Shin, Y.S., Lee, M.J., Jung, S.J., Hyun, D.Y., Kim, Y.C., Seong, N.S., Cha, S.W., Hwang, B.: Enhanced production of asiaticoside from hairy root cultures of Centella asiatica (L.) Urban elicited by methyl jasmonate. - Plant Cell Rep. 26: 1941-1949, 2007. Go to original source...
    7. Kim, O.T., Kim, M.Y., Huh, S.M., Bai, D.G., Ahn, J.C., Hwang, B.: Cloning of a cDNA probably encoding oxidosqualene cyclase associated with asiaticoside biosynthesid from Centella asiatica (L.) Urban. - Plant Cell Rep. 23: 304-311, 2005a. Go to original source...
    8. Kim, O.T., Kim, M.Y., Hwang, S.J., Ahn, J.C., Hwang, B.: Cloning and molecular analysis of cDNA encoding cycloartenol synthase from Centella asiatica (L.) Urban. - Biotechnol Bioprocess Eng. 10: 16-22, 2005b. Go to original source...
    9. Kim, O.T., Seong, N.S., Kim, M.Y., Hwang, B.: Isolation and characterization of squalene synthase cDNA from Centella asiatica (L.) Urban. - J. Plant. Biol. 48: 263-269, 2005c. Go to original source...
    10. Laus, G.: Advances in chemistry and bioactivity of the genus Uncaria. - Phytother. Res. 18: 259-274, 2004. Go to original source...
    11. Li, W., Dai, R.J., Yu, Y.H., Li, L., Meng, W.W., Deng, Y.L.: Two new flavonoid diglycosides from Cephalotaxus sinensis. - Chin. chem. Lett. 18: 837-839, 2007. Go to original source...
    12. Lichtenthaler, H.K.: Discovery of the two parallel pathways for isoprenoid biosynthesis in plants. -In: Kung S-D, Yang S.F. (ed.): Discoveries in Plant Biology. Pp. 141-161. World Scientific, River Edge - Singapore 2001. Go to original source...
    13. Liu, Y.L., Cai, Y.F., Zhao, Z.J., Wang, J.F., Li, J., Xin, W., Xia, G.M., Xiang, F.N.: Cloning and functional analysis of a β-amyrin synthase gene associated with oleanolic acid biosynthesis in Gentiana straminea Maxim. - Biol. Pharm. Bull. 32: 818-824, 2009. Go to original source...
    14. Liu, Z.J.: Drought-induced in vivo synthesis of camptothecin in Camptotheca acuminata seedlings. - Physiol. Plant. 110: 483-488, 2008. Go to original source...
    15. Magdi, E.S., Rob, V.: Catharanthus terpenoid indole alkaloids: biosynthesis and regulation. - Phytochem. Rev. 6: 277-305, 2007. Go to original source...
    16. Mangas, S., Moyano, E., Osuna, L., Cusido, R.M., Bonfill, M., Palazón, J.: Triterpenoid saponin content and the expression level of some related genes in calli of Centella asiatica. - Biotechnol. Lett. 30: 1853-1859, 2008. Go to original source...
    17. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays for tobacco tissue cultures. - Physiol. Plant. 15: 473-497, 1962. Go to original source...
    18. Nims, E., Dubois, C.P., Roberts, S.C., Walker, E. L.: Expression profiling of genes involved in paclitaxel biosynthesis for targeted metabolic engineering. - Metabolism Eng. 8: 385-394, 2006. Go to original source...
    19. Tan, R.X., Kong, L.D.: Secoiridoids from Gentiana siphonantha. - Phytochemistry 46: 1035-1038, 1997. Go to original source...
    20. Wang, X., Seed, B.: A PCR primer bank for quantitative gene expression analysis. - Nucl. Acids Res. 31: 1-8, 2003. Go to original source...
    21. Zindorn, C.: Altitudinal variation of secondary metabolites in flowering heads of the Asteraceae: trends and causes. - Phytochem. Rev. 9: 197-203, 2009. Go to original source...
    22. Zobayed, S.M.A., Afreen, F., Kozai, T.: Temperature stress can alter the photosynthetic efficiency and secondary metabolite concentrations in St. John's wort. - Plant Physiol. Biochem. 43: 977-984, 2005 Go to original source...

    Return to the content