biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:219-223, 2013 | DOI: 10.1007/s10535-012-0272-2

Methylglyoxal with glycine or succinate enhances differentiation and shoot morphogenesis in Nicotiana tabacum callus

A. Ray1, S. Ray2, S. Mukhopadhyay3, M. Ray4,*
1 Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata, India
2 Kolkata, India
3 Department of Botany, University of Calcutta, Kolkata, India
4 Department of Molecular Medicine, Bose Institute, Kolkata, India

The aim of this study was to evaluate the influence of methylglyoxal (MG) on organogenesis and regeneration of tobacco (Nicotiana tabacum L.) plants from callus in media containing glycine or succinate. The best improvement in shoot proliferation and shoot length was obtained in the medium supplemented with 0.1 mM MG and 0.5 mM glycine or 0.25 mM succinate. The histological studies showed vigorous development of corm like structures and shoot organogenesis from callus tissues cultured in MG supplemented media. Biochemical studies also revealed higher content of δ-aminolaevulinic acid (a precursor of chlorophyll) and of chlorophyll.

Keywords: δ-aminolevulinic acid; benzyladenine; chlorophyll; regeneration; tobacco
Subjects: δ-aminolevulinic acid; benzyladenine; cytokinins; chlorophyll; in vitro culture; tobacco; methylglyoxal; glycine; succinate; shoot regeneration

Received: November 28, 2011; Accepted: June 1, 2012; Published: June 1, 2013  Show citation

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Ray, A., Ray, S., Mukhopadhyay, S., & Ray, M. (2013). Methylglyoxal with glycine or succinate enhances differentiation and shoot morphogenesis in Nicotiana tabacum callus. Biologia plantarum, 57(2), 219-223. doi: 10.1007/s10535-012-0272-2
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