Photosynthetica 2006, 44(1):143 | DOI: 10.1007/s11099-005-0171-2

Photosynthetic responses and proline content of mature and young leaves of sunflower plants under water deficit

I. Cechin1,*, S. C. Rossi1, V. C. Oliveira1, T. F. Fumis1
1 Department of Biology, Faculty of Science, Sao Paulo State University, Bauru-SP, Brazil

In mature and young leaves of sunflower (Helianthus annuus L. cv. Catissol-01) plants grown in the greenhouse, photosynthetic rate, stomatal conductance, and transpiration rate declined during water stress independently of leaf age and recovered after 24-h rehydration. The intercellular CO2 concentration, chlorophyll (Chl) content, and photochemical activity were not affected by water stress. However, non-photochemical quenching increased in mature stressed leaves. Rehydration recovered the levels of non-photochemical quenching and increased the Fv/Fm in young leaves. Drought did not alter the total Chl content. However, the accumulation of proline under drought was dependent on leaf age: higher content of proline was found in young leaves. After 24 h of rehydration the content of proline returned to the same contents as in control plants.

Additional key words: chlorophyll; drought; intercellular CO2 concentration; leaf water potential; non-photochemical quenching; photochemical activity; rehydration; stomatal conductance to water vapour; transpiration rate

Received: June 20, 2005; Accepted: August 31, 2005; Published: March 1, 2006  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Cechin, I., Rossi, S.C., Oliveira, V.C., & Fumis, T.F. (2006). Photosynthetic responses and proline content of mature and young leaves of sunflower plants under water deficit. Photosynthetica, 44(1), 143. doi: 10.1007/s11099-005-0171-2
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidases in Beta vulgaris. - Plant Physiol. 24: 1-15, 1949. Go to original source...
    2. Bajji, M., Lutts, S., Kinet, J.-M.: Water deficit effects on solute contribution to osmotic adjustment as a function of leaf ageing in three durum wheat (Triticum durum Desf.) cultivars performing differently in arid conditions. - Plant Sci. 160: 669-681, 2001. Go to original source...
    3. Bertamini, M., Nedunchezhian, N.: Leaf age effects on chlorophyll, Rubisco, photosynthetic electron transport activities and thylakoid membrane protein in field grown grapevine leaves. - J. Plant Physiol. 159: 799-803, 2002. Go to original source...
    4. Carceller, M., Dambrogio, A.: Growth and differentiation of callus-cultures of sunflower (Helianthus annuus L.) in response to NaCl and proline. - Phyton 55: 115-122, 1994.
    5. Claussen, W.: Proline as a measure of stress in tomato plants. - Plant Sci. 168: 241-248, 2005. Go to original source...
    6. Clifford, S.C., Arndt, S.K., Corlett, J.E., Joshi, S., Sankhla, N., Popp, M., Jones, H.G.: The role of solute accumulation, osmotic adjustment and changes in cell wall elasticity in drought tolerance in Ziziphus mauritiana (Lamk.). - J. exp. Bot. 49: 967-977, 1998. Go to original source...
    7. Cornic, G.: Drought stress inhibits photosynthesis by decreasing stomatal aperture - not by affecting ATP synthesis. - Trends Plant Sci. 5: 187-188, 2000. Go to original source...
    8. David, M.M., Coelho, D., Barrote, I., Correia, M.J.: Leaf age effects on photosynthetic activity and sugar accumulation in droughted and rewatered Lupinus albus plants. - Aust. J. Plant Physiol. 25: 299-306, 1998. Go to original source...
    9. Flexas, J., Escalona, J.M., Medrano, H.: Water stress induces different levels of photosynthesis and electron transport rate regulation in grapevines. - Plant Cell Environ. 22: 39-48, 1999. Go to original source...
    10. Flexas, J., Medrano, H.: Energy dissipation in C3 plants under drought. - Funct. Plant Biol. 29: 1209-1215, 2002a. Go to original source...
    11. Flexas, J., Medrano, H.: Drought-inhibition of photosynthesis in C3 plants: stomatal and non-stomatal limitations revisited. - Ann. Bot. 89: 183-189, 2002b. Go to original source...
    12. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    13. Gzik, A.: Accumulation of proline and pattern of α-amino acids in sugar beet plants in response to osmotic, water and salt stress. - Environ. exp. Bot. 36: 29-38, 1996. Go to original source...
    14. Haupt-Herting, S., Fock, H.P.: Oxygen exchange in relation to carbon assimilation in water-stressed leaves during photosynthesis. - Ann. Bot. 89: 851-859, 2002. Go to original source...
    15. Heckenberger, U., Roggatz, U., Schurr, U.: Effect of drought stress on the cytological status in Ricinus communis. - J. exp. Bot. 49: 181-189, 1998. Go to original source...
    16. Hewitt, E.J.: Sand and Water Culture Methods Used in the Study of Plant Nutrition. - Commonwealth Agricultural Bureaux, Farnham Royal 1966.
    17. Jones, M.M., Turner, N.C.: Accumulation of solutes in leaves of sorghum and sunflower in response to water deficits. - Aust. J. Plant Physiol. 7: 193-205, 1980. Go to original source...
    18. Maggio, A., Miyazaki, S., Veronese, P., Fujita, T., Ibeas, J.I., Damsz, B., Narasimhan, M.L., Hasegawa, P.M., Joly, R.J., Bressan, R.A.: Does proline accumulation play an active role in stress-induced growth reduction? - Plant J. 31: 699-712, 2002. Go to original source...
    19. Olsson, M.: Alterations in lipid-composition, lipid-peroxidation and antioxidative protection during senescence in drought-stressed plants and non-drought stressed plants of Pisum sativum. - Plant Physiol. Biochem. 33: 547-553, 1995.
    20. Pankovic, D., Sakac, Z., Kevresan, S., Plesnicar, M.: Acclimation to long-term water deficit in the leaves of two sunflower hybrids: photosynthesis, electron transport and carbon metabolism. - J. exp. Bot. 330: 127-138, 1999. Go to original source...
    21. Tang, A.-C., Kawamitsu, Y., Kanechi, M., Boyer, J.S.: Photosynthetic oxygen evolution at low water potential in leaf discs lacking an epidermis. - Ann. Bot. 89: 861-870, 2002. Go to original source...
    22. Tezara, W., Mitchell, V.J., Driscoll, S.D., Lawlor, D.W.: Water stress inhibits plant photosynthesis by decreasing coupling factor and ATP. - Nature 401: 914-917, 1999. Go to original source...
    23. Torrelo, W.A., Rice, L.A.: Effects of NaCl stress on proline and cation accumulation in salt sensitive tolerance turfgrass. - Plant Soil 39: 241-247, 1986. Go to original source...

    Return to the content