Seasonal distribution of loline alkaloid concentration in meadow fescue infected with Neotyphodium uncinatum
Brian Patchett A , Ravi Gooneratne A C , Lester Fletcher B and Bruce Chapman A
+ Author Affiliations
- Author Affiliations
A Faculty of Agriculture and Life Sciences, PO Box 94, Lincoln University, Canterbury 7647, New Zealand.
B AgResearch Ltd, Private Bag 4749, Christchurch 8140, New Zealand.
C Corresponding author. Email: Ravi.Gooneratne@lincoln.ac.nz
Crop and Pasture Science 62(7) 603-609 https://doi.org/10.1071/CP11017
Submitted: 28 January 2011 Accepted: 19 June 2011 Published: 28 July 2011
Abstract
Loline alkaloids are present in meadow fescue containing the endophyte (Neotyphodium uncinatum Gams, Petrini and Schmidt) (Clavicipitacae). Root, crown and shoot loline alkaloid concentrations in 10 selected lines from meadow fescue ecotypes are reported for the first time, from a Canterbury farm during 2004–05. The concentrations of four loline alkaloid derivatives, N-formyl loline (NFL), N-acetyl loline (NAL), N-acetyl norloline (NANL) and N-methyl loline (NML), in these lines (each line represented by one genotype) were determined at four harvest dates during late spring, late summer, and early and late autumn. There were marked differences in loline alkaloid concentration between lines and seasons. Maximum shoot loline concentration was recorded in summer (up to 2860 µg/g in Fp408). Root loline alkaloid concentration was substantially higher in late autumn (up to 790 µg/g in Fp408) and the shoot concentration correspondingly lower than in spring, summer and early autumn suggesting loline alkaloid transportation from shoots to roots. In the lines tested at each of the four harvest dates in spring, summer, and autumn, the root, crown, and shoot alkaloid concentration with minor exceptions was NFL > NAL > NANL > NML.
Additional keywords: Clavicipitacae, Festuca pratensis, larvae, N-formyl loline, Poaceae.
References
Baldwin IT (2001) An ecologically motivated analysis of plant–herbivore interactions in native tobacco. Plant Physiology 127, 1449–1458.| An ecologically motivated analysis of plant–herbivore interactions in native tobacco.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XjtVWgsw%3D%3D&md5=115315a666584db0dd1ee1114463ced4CAS |
Ball OJP, Tapper BA (1999) The production of loline alkaloids in artificial and natural grass/endophyte associations. Proceedings of the New Zealand Plant Protection Conference 52, 264–269.
Blankenship JD, Spiering MJ, Wilkinson HH, Fannin FF, Bush LP, Schardl CL (2001) Production of loline alkaloids by the grass endophyte Neotyphodium uncinatum, in defined media. Phytochemistry 58, 395–401.
| Production of loline alkaloids by the grass endophyte Neotyphodium uncinatum, in defined media.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXms1Ons7o%3D&md5=78624e804979794ba7baec931a5dadccCAS |
Burhan W (1984) Development of Acremonium coenophialum and accumulation of N-acetyl and N-formyl loline in tall fescue (Festuca arundinacea Schreber). MSc Thesis, University of Kentucky, Lexington, KY, USA.
Bush LP, Fannin FF, Siegel MR, Dahlman DL, Burton HR (1993) Chemistry, occurrence and biological effects of saturated pyrrolizidine alkaloids associated with endophyte–grass interactions. Agriculture, Ecosystems & Environment 44, 81–102.
| Chemistry, occurrence and biological effects of saturated pyrrolizidine alkaloids associated with endophyte–grass interactions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXlsFKis78%3D&md5=8f835424e8205b966e3bfb851e7a6b00CAS |
Cagas B, Fleiger M, Olsovska J (1999) Concentration of ergot alkaloids in Czech ecotypes of Lolium perenne and Festuca pratensis. Grass and Forage Science 54, 365–370.
| Concentration of ergot alkaloids in Czech ecotypes of Lolium perenne and Festuca pratensis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXpt1yntA%3D%3D&md5=165240715a60125cc4ba6d61e97762c6CAS |
Chapman RB (1984) Pasture pests. In ‘New Zealand pests and beneficial insects’. (Ed. RR Scott) pp. 119–142. (Lincoln University College of Agriculture: Lincoln, New Zealand)
Cook R, Lewis GC (2001) Fungal endophytes and nematodes of agricultural and amenity grasses. In ‘Biotic interactions in plant–pathogen associations’. (Eds MJ Jeger, NJ Spence) pp. 35–61. (CAB International: Wallingford, UK)
Dahlman DL, Eichenseer PA, Siegel MR (1991) Chemical perspectives on endophye–grass interactions and their implications to insect herbivory. In ‘Microbial mediation of plant herbivore interactions’. (Ed. P Barbosa) pp. 227–252. (John Wiley: New York)
Fletcher LR, Popay AJ, Stewart AV, Tapper BA (2000) Herbage and sheep production from meadow fescue with and without the endophyte Neotyphodium uncinatum. In ‘Proceedings of the 4th International Neotyphodium/Grass Interactions Symposium’. Soest, Germany. (Eds VH Paul, PD Dapprich) (Universitat – Gesamthochshule: Paderborn, Abteilung Soest, Germany)
Hinton DM, Bacon CW (1985) The distribution and ultrastructure of the endophyte of tall fescue. Canadian Journal of Botany 63, 36–42.
| The distribution and ultrastructure of the endophyte of tall fescue.Crossref | GoogleScholarGoogle Scholar |
Jensen JG, Popay AJ (2007) Reductions in root aphid populations by non-toxic endophyte strains in tall fescue. In ‘Proceedings of the 6th International Symposium on Fungal Endophytes of Grasses’. Grasslands Research and Practice Series No. 13. (Eds AJ Popay, ER Thom) pp. 341–344. (New Zealand Grassland Association: Dunedin, New Zealand)
Jensen JG, Popay AJ, Tapper BA (2009) Argentine stem weevil adults are affected by meadow fescue endophyte and its loline alkaloids. New Zealand Plant Protection 62, 12–18.
Justus M, Witte L, Hartmann T (1997) Levels and tissue distribution of loline alkaloids in endophyte-infected Festuca pratensis. Phytochemistry 44, 51–57.
| Levels and tissue distribution of loline alkaloids in endophyte-infected Festuca pratensis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXhtl2ntQ%3D%3D&md5=8fe790049b273eea32722e5f9a059f92CAS |
Latch GCM, Hunt WF, Musgrave DR (1985) Endophytic fungi affect growth of perennial ryegrass. New Zealand Journal of Agricultural Research 28, 165–168.
Leuchtmann A, Schmidt D, Bush LP (2000) Different levels of protective alkaloids in grasses with stroma-forming and seed-transmitted Epichloe/Neotyphodium endophytes. Journal of Chemical Ecology 26, 1025–1036.
| Different levels of protective alkaloids in grasses with stroma-forming and seed-transmitted Epichloe/Neotyphodium endophytes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXisl2gs70%3D&md5=f75eddd747ec1b50840657a10c7b04a2CAS |
Patchett BJ (2007) Loline alkaloids: analysis and effects on sheep and pasture insects. PhD Thesis, Lincoln University, Canterbury, New Zealand.
Patchett BJ, Chapman RB, Fletcher LR, Gooneratne SR (2008) Root loline concentration in endophyte-infected meadow fescue (Festuca pratensis) is increased by grass grub (Costelytra zealandica) attack. New Zealand Plant Protection 61, 210–214.
Patterson CG, Potter DA, Fannin FF (1991) Feeding deterrency of alkaloids from endophyte-infected grasses to Japanese beetle (Coleoptera: Scarabaeidae) grubs. Entomologia Experimentalis et Applicata 61, 285–289.
| Feeding deterrency of alkaloids from endophyte-infected grasses to Japanese beetle (Coleoptera: Scarabaeidae) grubs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38XhsVylsr0%3D&md5=b28d4d7ed95dd9f7eee30c01ce9d7774CAS |
Payne RW, Murray DA, Harding SA, Baird DB, Soutar DM (2007) ‘Genstat for Windows: Introduction.’ 10th edn (VSN International: Hemel Hempstead, UK)
Pennell CG, Ball OJP (1999) Effects of Neotyphodium endophytes in tall fescue on pasture mealy bug (Bolanococcus poae). Proceedings of the New Zealand Plant Protection Conference 52, 259–263.
Petroski RJ, Yates SG, Weisleder D, Powell RG (1989) Isolation, semisynthesis and NMR spectral studies of loline alkaloids. Journal of Natural Products 52, 810–817.
| Isolation, semisynthesis and NMR spectral studies of loline alkaloids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXlt1WrsA%3D%3D&md5=a7f1faf229cd5401966e42a133b1f84fCAS |
Popay AJ, Bonos SA (2005) Biotic responses in endophytic grasses. In ‘Neotyphodium in cool-season grasses’. (Eds CA Roberts, CP West, DE Spiers) pp. 163–185. (Blackwell Publishing: Ames, IA)
Popay AJ, Lane GA (2000) The effect of crude extracts containing loline alkaloids on two New Zealand insect pests. In ‘Proceedings of the 4th International Neotyphodium/Grass Interactions Symposium’. Soest, Germany. (Eds VH Paul, PD Dapprich) pp. 471–476. (Universitat – Gesamthochshule: Paderborn, Abteilung Soest, Germany)
Popay AJ, Hume DE, Davis KL, Tapper BA (2003) Interactions between endophyte (Neotyphodium spp.) and ploidy in hybrid and perennial ryegrass cultivars and their effects on Argentine stem weevil (Listronotus bonariensis). New Zealand Journal of Agricultural Research 46, 311–319.
| Interactions between endophyte (Neotyphodium spp.) and ploidy in hybrid and perennial ryegrass cultivars and their effects on Argentine stem weevil (Listronotus bonariensis).Crossref | GoogleScholarGoogle Scholar |
Popay AJ, Tapper BA, Podmore C (2009) Endophyte-infected meadow fescue and loline alkaloids affect Argentine stem weevil larvae. New Zealand Plant Protection 62, 19–27.
Potter DA, Patterson CG, Redmond CT (1992) Influence of turfgrass species and tall fescue endophyte on feeding of Japanese beetle and Southern masked chafer grubs (Coleoptera: Scarabaeidae). Journal of Economic Entomology 85, 900–909.
Riedell WE, Kieckhefer RE, Petroski RJ, Powell RG (1991) Naturally-occurring and synthetic loline alkaloid derivatives: insect feeding behaviour modification and toxicity. Journal of Entomological Science 26, 122–129.
Salminen SO, Grewal PS (2002) Does decreased mowing frequency enhance alkaloid production in endophytic tall fescue and perennial ryegrass. Journal of Chemical Ecology 28, 939–950.
| Does decreased mowing frequency enhance alkaloid production in endophytic tall fescue and perennial ryegrass.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XktFSmtrY%3D&md5=fa6095e441c5cf3652c57e2ab66008e5CAS |
Schardl CL, Leutchmann A, Spiering MJ (2004) Symbioses of grasses with seedborne fungal endophytes. Annual Review of Plant Biology 55, 315–340.
| Symbioses of grasses with seedborne fungal endophytes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXlvFeisL8%3D&md5=f83ce34602aa07587a81b7c8daf40f08CAS |
Yates SG, Petroski RJ, Powell RG (1990) Analysis of loline alkaloids in endophyte-infected tall fescue by capillary gas chromatography. Journal of Agricultural and Food Chemistry 38, 182–185.
| Analysis of loline alkaloids in endophyte-infected tall fescue by capillary gas chromatography.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXltVSltQ%3D%3D&md5=434dcb2a257da9b0f54b4f4d59b9e7d5CAS |
