1887

Abstract

SUMMARY

Messenger RNA (mRNA) with sequences of polyriboadenylic acid (poly A) has been isolated from the cytoplasm of vaccinia virus-infected cells after a short pulse label with radioactive uridine. No labelled RNA containing poly A was isolated from mock-infected cells under the same conditions. mRNA synthesized at various times during the replicative cycle was examined by polyacrylamide gel electrophoresis and compared with the mRNA synthesized in the presence of inhibitors of protein and DNA synthesis. No difference was detected between ‘class 1’ and ‘class 2 early’ mRNA species although ‘late’ RNA species were on average considerably larger than the ‘early’ species.

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1974-02-01
2024-05-01
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References

  1. Atherton K. T., Darby G. 1974; Extraction of RNA from mammalian tissue culture cells. Analytical Biochemistry (in the press)
    [Google Scholar]
  2. Aviv H., Leder P. 1972; Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proceedings of the National Academy of Sciences of the United States of America 69:1408–1412
    [Google Scholar]
  3. Beale A. J., Christofinis G. C., Furminger J. G. S. 1963; Rabbit cells susceptible to Rubella virus. Lancet 11:640–641
    [Google Scholar]
  4. Borun T. W., Scharff M. D., Robbins E. 1967; Preparation of mammalian polyribosomes with the detergent Nonidex P-40. Biochimica et biophysica acta 149:302–304
    [Google Scholar]
  5. Brawerman G., Mendecki J., Lee S. Y. 1972; A procedure for the isolation of mammalian messenger ribonucleic acid. Biochemistry 11:637–641
    [Google Scholar]
  6. Dales S. 1963; The uptake and development of vaccinia virus in strain L cells followed with labelled viral DNA. Journal of Cell Biology 18:51–72
    [Google Scholar]
  7. Esteban M., Metz D. H. 1973; Early protein synthesis in vaccinia virus-infected cells. Journal of General Virology 19:201–216
    [Google Scholar]
  8. Gillespie D., Spiegelman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilised on a membrane. Journal of Molecular Biology 12:829–842
    [Google Scholar]
  9. House W., Wildy P. 1965; Large scale production of tissue cells and virus. Laboratory Practice 14:594–595
    [Google Scholar]
  10. Joklik W. K. 1962; Some properties of poxvirus deoxyribonucleic acid. Journal of Molecular Biology 5:265–274
    [Google Scholar]
  11. Joklik W. K., Jungwirth C., Oda K., Woodson B. 1967; Early and late functions during the vaccinia virus multiplication cycle. In The Molecular Biology of Viruses pp. 473–494 Edited by Coleter J. S., Parenchych W. New York: Academic Press;
    [Google Scholar]
  12. Kates J. 1970; Transcription of the vaccinia virus genome and the occurrence of polyriboadenylic acid sequences in messenger RNA. Cold Spring Harbor Symposia on Quantitative Biology 35:743–752
    [Google Scholar]
  13. Kates J., Beeson J. 1970; Ribonucleic acid synthesis in vaccinia virus. I. The mechanism of synthesis and release of RNA in vaccinia cores. Journal of Molecular Biology 50:1–18
    [Google Scholar]
  14. Kates J., Mcauslan B. R. 1967; Messenger RNA synthesised by a ‘coated’ viral genome. Proceedings of the National Academy of Sciences of the United States of America 58:134–141
    [Google Scholar]
  15. McAuslan B. R. 1963; Control of induced thymidine kinase in the poxvirus-infected cell. Virology 20:162–168
    [Google Scholar]
  16. Oda K., Joklik W. K. 1967; Hybridisation and sedimentation studies on ‘early’ and ‘late’ vaccinia messenger RNA. Journal of Molecular Biology 27:395–419
    [Google Scholar]
  17. Peacock A. C., Dingman C. W. 1968; Molecular weight estimation and separation of RNA by electrophoresis in agarose-acrylamide composite gels. Biochemistry 7:668–674
    [Google Scholar]
  18. Pennington T. H. 1973; Vaccinia virus morphogenesis: a comparison of virus-induced antigens and polypeptides. Journal of General Virology 19:65–79
    [Google Scholar]
  19. Salzman N. P., Shatkin A. J., Sebring A. D. 1964; The synthesis of a DNA-like RNA in the cytoplasm of HeLa cells infected with vaccinia virus. Journal of Molecular Biology 8:405–416
    [Google Scholar]
  20. Sarov I., Becker Y. 1967; Studies on vaccinia virus DNA. Virology 33:369–375
    [Google Scholar]
  21. Vantsis J. T., Wildy P. 1962; Interaction of herpes virus and HeLa cels: Comparison of cell killing and infective centre formation. Virology 17:225–232
    [Google Scholar]
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