1887

Abstract

Summary

The ribonucleoprotein (RNP) of avian infectious bronchitis virus (IBV) was examined by electron microscopy after shadowing with carbon/platinum. Linear RNP strands up to 6.7 µm in length, from three IBV strains, were sensitive to both pancreatic RNase and to proteases. These strands were obtained from spontaneously disrupted complete particles but not from disrupted incomplete particles that lacked RNP. They were also released from Nonidet P40-disrupted particles and could be isolated on sucrose density gradients at a density of 1.27 g/ml. In some cases, helical RNP complexes associated with virus particles were observed that were similar to RNPs of human coronavirus strain 229E and mouse hepatitis virus strain 3.

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1981-03-01
2024-05-01
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References

  1. Alexander D. J., Coluns M. S. 1977; The purification and polypeptide composition of avian infectious bronchitis virus. Microbios 18:87–98
    [Google Scholar]
  2. Apostolov K., Flewett T. H., Kendal A. P. 1970; Morphology of influenza A. B. C. and infectious bronchitis virus (IBV) virions and (heir replication. In The Biology of Large RNA Viruses pp 3–26 Edited by Barry R. D., Maliv B. W. J. London: Academic Press;
    [Google Scholar]
  3. Bingham R. 1975; The polypeptide composition of avian infectious bronchitis virus. Archives of Virology 49:207–216
    [Google Scholar]
  4. Caul E. O., Egglestone S. I. 1977; Further studies on human enteric coronaviruses. Archives of Virology 54:107–117
    [Google Scholar]
  5. Caul E. O., Ashley C. R., Ferguson M., Egglestone S. I. 1979; Preliminary studies on the isolation of coronavirus 229E nucleocapsids. FEMS Microbiology Letters 5:101–105
    [Google Scholar]
  6. Collins M. S., Alexander D. J., Harkness J. W. 1976; Heterogeneity of infectious bronchitis virus grown in eggs. Archives of Virology 50:55–72
    [Google Scholar]
  7. Kennedy D. A., Johnson-Lussenburg C. M. 1975/6; Isolation and morphology of the internal component of human coronavirus, strain 229E. Intervirology 6:197–206
    [Google Scholar]
  8. Lanser J. A., Howard C. R. 1980; The polypeptides of infectious bronchitis virus (IBV–41 strain). Journal of General Virology 46:349–361
    [Google Scholar]
  9. Lomniczi B., Kennedy I. 1977; Genome of infectious bronchitis virus. Journal of Virology 24:99–107
    [Google Scholar]
  10. McIntosh K. 1974; Coronaviruses: a comparative review. Current Topics in Microbiology and Immunology 63:85–129
    [Google Scholar]
  11. Macnaughton M. R. 1978; The genome of three coronaviruses. FEBS Letters 94:191–194
    [Google Scholar]
  12. Macnaughton M. R., Davies H. A. 1980; Two particle types of avian infectious bronchitis virus. Journal of General Virology 47:365–372
    [Google Scholar]
  13. Macnaughton M. R., Madge M. H. 1977a; The polypeptide composition of avian infectious bronchitis virus particles. Archives of Virology 55:47–54
    [Google Scholar]
  14. Macnaughton M. R., Madge M. H. 1977b; The characterisation of the virion RNA of avian infectious bronchitis virus. FEBS Letters 77:311–313
    [Google Scholar]
  15. Macnaughton M. R., Madge M. H., Davies H. A., Dourmashkin R. R. 1977; Polypeptides of the surface projections and the ribonucleoprotein of avian infectious bronchitis virus. Journal of Virology 24:821–824
    [Google Scholar]
  16. Macnaughton M. R., Davies H. A., Nermut M. V. 1978; Ribonucleoprotein-like structures from coronavirus particles. Journal of General Virology 39:545–549
    [Google Scholar]
  17. Nagy E., Lomniczi B. 1979; Polypeptide patterns of infectious bronchitis virus serotypes fall into two categories (1979). Archives of Virology 61:341–345
    [Google Scholar]
  18. Schochetman G., Stevens R. H., Simpson R. W. 1977; Presence of infectious polyadenyiated RNA in the coronavirus avian infectious bronchitis virus. Virology 77:772–782
    [Google Scholar]
  19. Tyrrell D. A. J., Alexander D. J., Almeida J. D., Cunningham C. H., Easterday B. C., Garwes D. J., Hierholzer J. C., Kapikian A., Macnaughton M. R., Mcintosh K. 1978; Coronaviridae: second report. Intervirology 10:321–328
    [Google Scholar]
  20. Watkins G., Reeve P., Alexander D. J. 1975; The ribonucleic acid of infectious bronchitis virus. Archives of Virology 47:279–286
    [Google Scholar]
  21. Watson D. H., Russell W. C., Wildy P. 1963; Electron microscopic particle counts on herpes virus using the phosphotungstate negative staining technique. Virology 19:250–260
    [Google Scholar]
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