1887

Abstract

The movement proteins (MP) of cowpea mosaic virus and cauliflower mosaic virus (CaMV) are associated with tubular structures which participate in the transmission of virus particles from cell to cell. Both proteins have been expressed in plant protoplasts and insect cells. In all cases, immunofluorescent histochemistry showed that the MPs accumulate intracellularly as tubular extensions projecting from the cell surface. Additionally, electron microscopy revealed intracellular MP aggregates in CaMV MP-expressing cells. The data presented establish common features for the tubule-forming MPs: no other virus gene products are required for tubule formation and unique plant components (e.g. plasmodesmata) are not essential for tubule synthesis.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-11-2857
1996-11-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/11/JV0770112857.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-11-2857&mimeType=html&fmt=ahah

References

  1. Brown M., Faulkner P. 1977; A plaque assay for nuclear polyhedrosis viruses using a solid overlay. Journal of General Virology 36:361–364
    [Google Scholar]
  2. Citovsky V., Knorr D., Zambryski P. 1991; Gene I, a potential cell-to-cell movement locus of cauliflower mosaic virus, encodes an RNA binding protein. Proceedings of the National Academy of Sciences, USA 88:2476–2480
    [Google Scholar]
  3. Damm B., Willmitzer L. 1988; Regeneration of fertile plants from protoplasts of different Arabidopsis thaliana genotypes. Molecular and General Genetics 213:15–20
    [Google Scholar]
  4. Damm B., Schmidt R., Willmitzer L. 1989; Efficient transformation of Arabidopsis thaliana using direct gene transfer to protoplasts. Molecular and General Genetics 217:6–12
    [Google Scholar]
  5. Groebe D. R., Chung A. E., Ho C. 1990; Cationic lipid-mediated cotransfection of insect cells. Nucleic Acids Research 18:4033
    [Google Scholar]
  6. Harker C. L., Mullineaux P. M., Bryant J. A., Maule A. J. 1987; Detection of CaMV gene I and gene VI products in vivo using antisera raised to COOH-terminal β-galactosidase fusion proteins. Plant Molecular Biology 8:275–287
    [Google Scholar]
  7. Hink F. 1970; Established insect cell line from the cabbage looper, Trichoplusia ni. Nature 226:446–467
    [Google Scholar]
  8. Jones J. D. G., Shlumukov L., Carland F., English J., Scofield S. R., Bishop G. J., Harrison K. 1992; Effective vectors for transformation, expression of heterologous genes and assaying transposon excision in transgenic plants. Transgenic Research 1:285–297
    [Google Scholar]
  9. Karesch H., Bilang R., Potrykus I. 1991a; Arabidopsis thaliana: protocol for plant regeneration from protoplasts. Plant Cell Reports 9:575–578
    [Google Scholar]
  10. Karesch H., Bilang R., Mittelsten-Scheid O. M., Potrykus I. 1991b; Direct gene transfer to protoplasts of Arabidopsis thaliana . Plant Cell Reports 9:571–574
    [Google Scholar]
  11. Kasteel D., Wellink J., Verver J., van Lent J., Goldbach R., van Kammen A. 1993; The involvement of cowpea mosaic virus M RNA-encoded proteins in tubule formation. Journal of General Virology 74:1721–1724
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  13. Linstead P. J., Hills G. J., Plaskitt K. A., Wilson I. G., Harker C. L., Maule A. J. 1988; The subcellular location of the gene 1 product of cauliflower mosaic virus is consistent with a function associated with virus spread. Journal of General Virology 69:1809–1818
    [Google Scholar]
  14. Lucas W. J., Gilbertson R. L. 1994; Plasmodesmata in relation to viral movement within leaf tissues. Annual Review of Phytopathology 32:387–411
    [Google Scholar]
  15. Martens J. W. M., van Oers M. M., van de Bilt B. D., Oudshoorn P., Vlak J. M. 1994; Development of a baculovirus that facilitates the generation of p10-based recombinants. Journal of Virological Methods 52:15–19
    [Google Scholar]
  16. Maule A. J. 1991; Virus movement in infected plants. Critical Reviews in Plant Science 9:457–473
    [Google Scholar]
  17. Maule A. J., Usmany M., Wilson I. G., Boudazin G., Vlak J. M. 1992; Biophysical and biochemical properties of baculovirus-expressed CaMV P1 protein. Virus Genes 6:5–18
    [Google Scholar]
  18. O’Reilly D. R., Miller L. K., Luckow V. A. 1992; Baculovirus Expression Vectors. A Laboratory Manual New York: W. H. Freeman;
    [Google Scholar]
  19. Perbal M. C., Thomas C. L., Maule A. J. 1993; Cauliflower mosaic virus gene I product (P1) forms tubular structures which extend from the surface of infected protoplasts. Virology 195:281–285
    [Google Scholar]
  20. Ritzenthaler C., Schmidt A.-C., Michler P., Stussi-Garaud C., Pinck L. 1995; Grapevine fanleaf nepovirus putative movement protein is involved in tubule formation in vivo . Molecular Plant–Microbe Interactions 8:379–387
    [Google Scholar]
  21. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Shanks M., Tomenius K., Clapham D., Huskisson N. S., Barker P. J., Wilson I. G., Maule A. J., Lomonossoff G. P. 1989; Identification and subcellular localization of a putative cell-to-cell transport protein from red clover mottle virus. Virology 173:400–407
    [Google Scholar]
  23. Storms M. M. H., Kormelink R., Peters D., van Lent J. W. M., Goldbach R. W. 1995; The nonstructural NSm protein of tomato spotted wilt virus induces tubular structures in plant and insect cells. Virology 214:485–493
    [Google Scholar]
  24. Thomas C. L., Maule A. J. 1995a; Identification of the cauliflower mosaic virus movement protein RNA-binding domain. Virology 206:1145–1149
    [Google Scholar]
  25. Thomas C. L., Maule A. J. 1995b; Identification of structural domains within the cauliflower mosaic vims movement protein by scanning deletion mutagenesis and epitope tagging. Plant Cell 7:561–572
    [Google Scholar]
  26. Thomas C. L., Perbal C., Maule A. J. 1993; A mutation of cauliflower mosaic vims gene I interferes with vims movement but not vims replication. Virology 192:415–421
    [Google Scholar]
  27. Van Lent J., Wellink J., Goldbach R. 1990a; Evidence for the involvement of the 58K and 48K proteins in the intracellular movement of cowpea mosaic vims. Journal of General Virology 71:219–223
    [Google Scholar]
  28. Van Lent J. W. M., Groenen J. T. M., Klinge-Roode E. C., Rohrman G. F., Zuidema D., Vlak J. M. 1990b; Localization of the 34 kDa Polyhedron envelope protein in Spodoptera frugiperda cells infected with Autographa califomica nuclear polyhedrosis vims. Archives of Virology 111:103–114
    [Google Scholar]
  29. Van Lent J., Storms M., van der Meer F., Wellink J., Goldbach R. 1991; Tubular structures involved in movement of cowpea mosaic vims are also formed in infected cowpea protoplasts. Journal of General Virology 72:2615–2623
    [Google Scholar]
  30. Vaughn J. L., Goodwin R. H., Tompkins G. J., McCawiey P. 1977; The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13:312–317
    [Google Scholar]
  31. Vlak J. M., Schouten A., Usmany M., Belsham G. J., Klinge-Roode E. C., Maule A. J., van Lent J. W. M., Zuidema D. 1990; Expression of cauliflower mosaic vims gene I using a baculovims vector based upon the p10 gene and a novel selection method. Virology 179:312–320
    [Google Scholar]
  32. Wellink J., Jaegle M., Prinz H., van Kammen A., Goldbach R. 1987; Expression of the middle component RNA of cowpea mosaic vims in vivo . Journal of General Virology 68:2577–2585
    [Google Scholar]
  33. Wellink J., van Lent J. W. M., Verver J., Sijen T., Goldbach R. W., van Kammen A. 1993; The cowpea mosaic vims M RNA-encoded 48-Kilodalton protein is responsible for induction of tubular structures in protoplasts. Journal of Virology 67:3660–3664
    [Google Scholar]
  34. Wiecorek A., Sanfacon H. 1993; Characterization and subcellular location of tomato ringspot nepovirus putative movement protein. Virology 194:734–742
    [Google Scholar]
  35. Wolf S., Deom C. M., Beachy R. N., Lucas W. J. 1989; Movement protein of tobacco mosaic virus modifies plasmodesmatal size exclusion limit. Science 246:377–379
    [Google Scholar]
  36. Zuidema D., Schouten A., Usmany M., Maule A. J., Belsham G. J., Roosien J., Klinge-Roode E. C., VanLent J. W. M., Vlak J. M. 1990; Expression of cauliflower mosaic virus gene I in insect cells using a novel polyhedrin-based baculovirus expression vector. Journal of General Virology 71:2201–2209
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-11-2857
Loading
/content/journal/jgv/10.1099/0022-1317-77-11-2857
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error