1887

Abstract

Maize streak virus (MSV) coat protein (CP) is required for virus movement within the plant. Deletion or mutation of MSV CP does not prevent virus replication in single cells or protoplasts but leads to a loss of infectivity in the inoculated plant. The mechanism by which MSV CP mediates the transfer of MSV DNA from cell to cell and through the vascular bundle is still unknown. Towards understanding the role of MSV CP in virus movement, the interaction of the CP with viral DNA was investigated using the ‘south-western’ assay. Wild-type and truncated MSV CPs were expressed in and the expressed CPs were used to investigate interactions with single-stranded (ss) and double-stranded (ds) DNA. The results showed that MSV CP bound ss and ds viral and plasmid DNA in a sequence non-specific manner. The binding domain was mapped to within the 104 N-terminal amino acids of the MSV CP. We propose that the binding of CP to MSV DNA is involved in viral DNA nuclear transport as well as encapsidation and thus may have a role in intra- and inter-cellular movement as well as systemic infection.

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1997-06-01
2024-04-28
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References

  1. Bock K. R., Guthrie E. J., Woods R. G. 1974; Purification of maize streak virus and its relationship to virus associated with streak diseases of sugar cane and Panicum maximum. Annals of Applied Biology 77:289–296
    [Google Scholar]
  2. Boulton M. I., Steinkellner H., Donson J., Markham P. G., King D. I., Davies J. W. 1989; Mutational analysis of the virion-sense genes of maize streak virus. Journal of General Virology 70:2309–2323
    [Google Scholar]
  3. Boulton M. I., King D. I., Donson J., Davies J. W. 1991; Point substitutions in a promoter-like region and the V1 gene affect the host range and symptoms of maize streak virus. Virology 183:114–121
    [Google Scholar]
  4. Boulton M. I., Pallaghy C. K., Chatani M., MacFarlane S., Davies J. W. 1993; Replication of maize streak virus mutants in maize protoplasts: evidence for a movement protein. Virology 192:85–93
    [Google Scholar]
  5. Brakke M. K. 1990; Preparation of antigens, viruses. In Serological Methodsfor Detection and Identification of Viral and Bacterial Plant Pathogens pp. 15–26 Hampton R., Ball E., De Boer S. Edited by St Paul, Minn: APS press;
    [Google Scholar]
  6. Citovsky V., Knorr D., Zambryski P. 1991; Gene I, a potential cell-to-cell movement locus of cauliflower mosaic virus, encodes an RNA- binding protein. Proceedings of the National Academy of Sciences, USA 88:2476–2480
    [Google Scholar]
  7. Davies J. W., Stanley J., Donson J., Mullineaux P. M., Boulton M. I. 1987; Structure and replication of geminivirus genomes. Journal of Cell Science Supplement 7:95–107
    [Google Scholar]
  8. Dickinson V. J., Halder J., Woolston C. J. 1996; The product of maize streak virus ORF V1 is associated with secondary plasmodesmata and is first detected with the onset of viral lesions. Virology 220:51–59
    [Google Scholar]
  9. Gilbertson R. L., Lucas W. J. 1996; How do viruses traffic on the ‘vascular highway’?. Trends in Plant Science 1:260–268
    [Google Scholar]
  10. Goodman R. M. 1981; Geminiviruses. In Handbook of Plant Virus Infection and Comparative Diagnosis pp. 879–910 Kurstak E. Edited by New York: Elsevier/North Holland Biomedical Press;
    [Google Scholar]
  11. Guerineau F., Lucy A., Mullineaux P. 1992; Effect of two consensus sequences preceding the translation initiator codon on gene expression in plant protoplasts. Plant Molecular Biology 18:815–818
    [Google Scholar]
  12. Howell S. H. 1985; Physical structure and genetic organization of the genome of maize streak virus (Kenyan isolate). Nucleic Acids Research 13:3018–3019
    [Google Scholar]
  13. Ingham D. J., Pascal E., Lazarowitz S. G. 1995; Both geminivirus movement proteins define viral host range, but only BL1 determines viral pathogenicity. Virology 207:191–204
    [Google Scholar]
  14. Jeffrey J. L., Pooma W., Petty I. T. D. 1996; Genetic requirements for local and systemic movement of tomato golden mosaic virus in infected plants. Virology 223:208–218
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural protein during the assembly of head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  16. Lazarowitz S. G. 1988; Infectivity and complete nucleotide sequence of the genome of a South African isolate of maize streak virus. Nucleic Acids Research 16:229–249
    [Google Scholar]
  17. Lazarowitz S. G. 1992; Geminiviruses: genome structure andgenome function. Critical Reviews in Plant Sciences 11:327–349
    [Google Scholar]
  18. Lazarowitz S. G., Pinder A. J., Damsteegt V. D., Rogers S. G. 1989; Maize streak virus genes essential for systemic spread and symptom development. EMBO Journal 8:1023–1032
    [Google Scholar]
  19. Morris-Krsinich B. A. M., Mullineaux P. M., Donson J., Boulton M. I., Markham P. G., Short M. N., Davies J. W. 1985; Bidirectional transcription of maize streak virus DNA and identification of the coat protein gene. Nucleic Acids Research 13:7237–7256
    [Google Scholar]
  20. Mullineaux P. M., Donson J., Morris-Krsinich B. A. M., Boulton M. I., Davies J. W. 1984; The nucleotide sequence of maize streak virus DNA. EMBO Journal 3:3063–3068
    [Google Scholar]
  21. Mullineaux P. M., Boulton M. I., Bowyer P., van der Vlugt R., Marks M., Donson J., Davies J. W. 1988; Detection of a non-structural protein of MW 11000 encoded by the virion DNA of maize streak virus. Plant Molecular Biology 11:57–66
    [Google Scholar]
  22. Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D.editor 1995 Virus Taxonomy. Sixth report of the International Committee on Taxonomy of Viruses Vienna & New York: Springer-Verlag;
    [Google Scholar]
  23. Noueiry A. O., Lucas W. J., Gilbertson R. L. 1994; Two proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport. Cell 76:925–932
    [Google Scholar]
  24. Pascal E., Sanderfoot A. A., Ward B. M., Medville R., Turgeon R., Lazarowitz S. G. 1994; The geminivirus BR1 movement protein binds single-stranded DNA and localizes to the cell nucleus. Plant Cell 6:995–1006
    [Google Scholar]
  25. Pinner M. S., Markham P. G. 1990; Serotyping and strain identification of maize streak virus isolates. Journal of General Virology 71:1635–1640
    [Google Scholar]
  26. Pinner M. S., Medina V., Plaskitt K. A., Markham P. G. 1993; Viral inclusions in monocotyledons infected by maize streak and related viruses. Plant Pathology 42:75–87
    [Google Scholar]
  27. Pooma W., Gillette W. K., Jeffrey J. L., Petty I. T. D. 1996; Host and viral factors determine the dispensability of the coat protein for bipartite geminivirus systemic movement. Virology 218:264–268
    [Google Scholar]
  28. Robbins J., Dilworth S. M., Laskey R. A., Dingwall C. 1991; Two interdependent basic domains in nucleoplasmin nuclear targeting sequence : identification of a class of bipartite nuclear targeting sequences. Cell 64:615–623
    [Google Scholar]
  29. Sanderfoot A. A., Ingham D. J., Lazarowitz S. G. 1996; A viral movement protein as a nuclear shuttle. Plant Physiology 110:23–33
    [Google Scholar]
  30. Sigal N., Delius H., Kornberg T., Gefter M. L., Alberts B. 1972; A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases. Proceedings of the National Academy of Sciences, USA 69:3537–3541
    [Google Scholar]
  31. Studier F. W., Moffatt B. A. 1986; polymerase to direct selective high-level expression of cloned genes. Journal of Molecular Biology 189:113–130
    [Google Scholar]
  32. Sukegawa J., Blobel G. 1993; A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm. Cell 72:29–38
    [Google Scholar]
  33. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : procedure and some applications. Proceedings of the National Academy of Sciences, USA 76:4350–4354
    [Google Scholar]
  34. Varagona M. J., Raikhel N. V. 1994; The basic domain in the bZIP regulatory protein Opaque2 serves two independent functions: DNA binding and nuclear localization. Plant Journal 5:207–214
    [Google Scholar]
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