1887

Abstract

Summary

Of the total amount of staphylococcal a-toxin produced in batch cultures, 1 per cent. or less appeared to be cell-associated, indicating rapid release of the toxin into the environment. The level of cell-associated haemolysin reached its maximum at the onset of the stationary phase of growth and decreased thereafter. Cell-associated haemolysin was isolated by the combined use of gel filtration and iso-electric focusing and was characterised by several criteria. Purified material closely resembled the extracellular form of staphylococcal a-toxin.

The authors gratefully acknowledge the photographic assistance of Mr Ian McKee. This work was supported in part by grants from The Royal Society and the Scottish Home and Health Department.

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/content/journal/jmm/10.1099/00222615-5-1-123
1972-02-01
2024-04-25
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References

  1. Bernheimer A. W., Schwartz Lois L. 1963; Isolation and composition of staphylococcal alpha toxin. J Gen Microbiol 30:455
    [Google Scholar]
  2. Hendricks C. W., Altenbern R. A. 1968; Studies on the synthesis of staphylococcal alpha toxin. Canad J Microbiol 14:1277
    [Google Scholar]
  3. Lowry O. H., Rosebrough Nira J., Farr A. L., Randall Rose J. 1951; Protein measurement with the Folin phenol reagent. J Biol Chem 193:265
    [Google Scholar]
  4. McNiven A., Christine Owen P., Arbuthnott J. P. 1972; Multiple forms of staphylococcal alpha-toxin. J Med Microbiol 5:113
    [Google Scholar]
  5. Raynaud M., Alouf J. E. 1970 Intracellular versus extracellular toxins. In Microbial toxins, vol. 1, Bacterial protein toxins, ed. by Ajl S. J., Kadis S., Montie T. C. London and New York67
    [Google Scholar]
  6. Raynaud M., Turpin A., Mangalo R., Bizzini B. 1955; Croissance et toxinogenese. Annls Inst Pasteur Paris 88:24
    [Google Scholar]
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