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Volume 63, Issue Pt_6

sp. nov., an archaeon from deep-sea hydrothermal sediment Free

Abstract

A hyperthermophilic, anaerobic, piezophilic archaeon (strain DY20341) was isolated from a sediment sample collected from an East Pacific Ocean hydrothermal field (1° 37′ S 102° 45′ W) at a depth of 2737 m. The cells were irregular cocci, 0.8–1.5 µm in diameter. Growth was observed between 50 and 90 °C (optimum 80 °C), pH 5.0 and 8.0 (optimum pH 7.0), 1 % and 7 % (w/v) sea salts (Sigma, optimum 3 %), 1 % and 4 % (w/v) NaCl (optimum 3 %) and 0.1 and 80 MPa (optimum 30 MPa). The minimum doubling time was 66 min at 30 MPa and 80 °C. The isolate was an obligate chemoorganoheterotroph, capable of utilizing complex organic compounds and organic acids including yeast extract, peptone, tryptone, casein, starch, Casamino acids, citrate, lactate, acetate, fumarate, propanoate and pyruvate for growth. It was strictly anaerobic and facultatively dependent on elemental sulfur or sulfate as electron acceptors, but did not reduce sulfite, thiosulfate, Fe(III) or nitrate. The presence of elemental sulfur enhanced growth. The G+C content of the genomic DNA was 43.6±1 mol%. 16S rRNA gene sequence analysis revealed that the closest relative of the isolated organism was DMJ (95.7 % 16S rRNA gene similarity). On the basis of its physiological properties and phylogenetic analyses, the isolate is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is strain DY20341 ( = JCM 17873 = DSM 24777).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41006100)
  • National Program on Key Basic Research Project (Award 2012CB417300)
  • International Sci & Tech Cooperation Program of China (Award 2010DFB23320)
  • Agence Nationale de la Recherche (Award 10 BLAN 1725 01)
  • Partenariat Hubert Curien (PHC) Programme Français de Coopération avec la Chine (PFCC) (Award 20970WC)
  • PHC Xu Guangqi collaboration (Award 27941TL)
© 2013 IUMS
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2013-06-01
2024-04-24
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Palaeococcus pacificus sp. nov., an archaeon from deep-sea hydrothermal sediment
Int J Syst Evol Microbiol 63, 2155 (2013); https://doi.org/10.1099/ijs.0.044487-0
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