1887

Abstract

A novel anaerobic, mesophilic, amino-acid-fermenting bacterium, designated strain CL-84, was isolated from the swine intestinal tract on mucin-based media. Cells were curved rods (0.8–1.2×3.5–5.0 µm), stained Gram-negative and were non-motile with no evidence of spores. Strain CL-84 produced acetate, propionate, formate and butyrate as the end products of metabolism when grown on serine. Optimum growth occurred at 39 °C and pH 6.5. The major cellular fatty acids were iso-C, iso-C 3-OH, iso-C and C, distinguishing strain CL-84 from closely related species. The DNA G+C content of strain CL-84 was 55.1 mol%. 16S rRNA gene sequence analysis showed that strain CL-84 shared 90–95 % similarity with characterized genera within the phylum , family Phylogenetic analysis showed that strain CL-84 was related to, but distinct from, Based on these findings, we propose that strain CL-84 represents a novel species of the genus . We further propose the name sp. nov. be designated for this species. The type strain is CL-84 ( = DSM 25858 = CCUG 62631). An emended description of the genus is provided.

Funding
This study was supported by the:
  • Agricultural Research Service
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2013-06-01
2024-03-29
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