1887

Abstract

We report the isolation of Australian strains of Bustos virus and Ngewotan virus, two insect-specific viruses in the newly identified taxon , originally isolated from Southeast Asian mosquitoes. Consistent with the expected insect-specific tropism of negeviruses, these isolates of Ngewotan and Bustos viruses, alongside the Australian negevirus Castlerea virus, replicated exclusively in mosquito cells but not in vertebrate cells, even when their temperature was reduced to 34 °C. Our data confirmed the existence of two structural proteins, putatively one membrane protein forming the majority of the virus particle, and one glycoprotein forming a projection on the apex of the virions. We generated and characterized 71 monoclonal antibodies to both structural proteins of the two viruses, most of which were neutralizing. Overall, these data increase our knowledge of negevirus mechanisms of infection and replication .

Funding
This study was supported by the:
  • Australian Research Council (Award DP120103994)
    • Principle Award Recipient: Roy A Hall
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2020-01-31
2024-03-28
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References

  1. Vasilakis N, Forrester NL, Palacios G, Nasar F, Savji N et al. Negevirus: a proposed new taxon of insect-specific viruses with wide geographic distribution. J Virol 2013; 87:2475–2488 [View Article]
    [Google Scholar]
  2. Auguste AJ, Carrington CVF, Forrester NL, Popov VL, Guzman H et al. Characterization of a novel Negevirus and a novel bunyavirus isolated from Culex (Culex) declarator mosquitoes in Trinidad. J Gen Virol 2014; 95:481–485 [View Article]
    [Google Scholar]
  3. Nabeshima T, Inoue S, Okamoto K, Posadas-Herrera G, Yu F et al. Tanay virus, a new species of virus isolated from mosquitoes in the Philippines. Journal of General Virology 2014; 95:1390–1395 [View Article]
    [Google Scholar]
  4. Kallies R, Kopp A, Zirkel F, Estrada A, Gillespie T et al. Genetic characterization of goutanap virus, a novel virus related to negeviruses, cileviruses and higreviruses. Viruses 2014; 6:4346–4357 [View Article]
    [Google Scholar]
  5. Kawakami K, Kurnia YW, Fujita R, Ito T, Isawa H et al. Characterization of a novel negevirus isolated from Aedes larvae collected in a subarctic region of Japan. Arch Virol 2016; 161:801–809 [View Article]
    [Google Scholar]
  6. Fujita R, Kuwata R, Kobayashi D, Bertuso AG, Isawa H et al. Bustos virus, a new member of the negevirus group isolated from a Mansonia mosquito in the Philippines. Arch Virol 2017; 162:79–88 [View Article]
    [Google Scholar]
  7. Nunes MRT, Contreras-Gutierrez MA, Guzman H, Martins LC, Barbirato MF et al. Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus. Virology 2017; 504:152–167 [View Article]
    [Google Scholar]
  8. O'Brien CA, McLean BJ, Colmant AMG, Harrison JJ, Hall-Mendelin S et al. Discovery and characterisation of Castlerea virus, a new species of Negevirus isolated in Australia. Evol Bioinform Online 2017; 13(10.1177_1176934317691269.xml:1176934317691269
    [Google Scholar]
  9. Charles J, Tangudu CS, Hurt SL, Tumescheit C, Firth AE et al. Detection of novel and recognized RNA viruses in mosquitoes from the Yucatan Peninsula of Mexico using metagenomics and characterization of their in vitro host ranges. J Gen Virol 2018; 99:1729–1738 [View Article]
    [Google Scholar]
  10. Wang Y, Guo X, Peng H, Lu Y, Zeng X et al. Complete genome sequence of a novel negevirus isolated from Culex tritaeniorhynchus in China. Arch Virol 2019; 164:907–911 [View Article]
    [Google Scholar]
  11. Moraes OS, Cardoso BF, Pacheco TA, Pinto AZL, Carvalho MS et al. Natural infection by Culex flavivirus in Culex quinquefasciatus mosquitoes captured in Cuiaba, Mato Grosso Mid-Western Brazil. Med Vet Entomol 2019
    [Google Scholar]
  12. Carapeta S, do Bem B, McGuinness J, Esteves A, Abecasis A et al. Negeviruses found in multiple species of mosquitoes from southern Portugal: isolation, genetic diversity, and replication in insect cell culture. Virology 2015; 483:318–328 [View Article]
    [Google Scholar]
  13. Hall RA, Bielefeldt-Ohmann H, McLean BJ, O'Brien CA, Colmant AM et al. Commensal viruses of mosquitoes: host restriction, transmission, and interaction with arboviral pathogens. Evol Bioinform Online 2016; 12:35–44
    [Google Scholar]
  14. Wang J, Wu J, Li N, Cao Y, He Y et al. A new Tanay virus isolated from mosquitoes in Guangxi, China. Arch Virol 2018; 163:3177–3180 [View Article]
    [Google Scholar]
  15. Kondo H, Chiba S, Maruyama K, Andika IB, Suzuki N. A novel insect-infecting virga/nege-like virus group and its pervasive endogenization into insect genomes. Virus research 2017
    [Google Scholar]
  16. Gorchakov RV, Tesh RB, Weaver SC, Nasar F. Generation of an infectious Negev virus cDNA clone. J Gen Virol 2014; 95:2071–2074 [View Article]
    [Google Scholar]
  17. O’Brien CA, Hobson-Peters J, Yam AWY, Colmant AMG, McLean BJ et al. Viral RNA intermediates as targets for detection and discovery of novel and emerging mosquito-borne viruses. PLoS Negl Trop Dis 2015; 9:e0003629 [View Article]
    [Google Scholar]
  18. Warrilow D, Watterson D, Hall RA, Davis SS, Weir R et al. A new species of mesonivirus from the Northern Territory, Australia. PLoS One 2014; 9:e91103 [View Article]
    [Google Scholar]
  19. McLean BJ, Hobson-Peters J, Webb CE, Watterson D, Prow NA et al. A novel insect-specific flavivirus replicates only in Aedes-derived cells and persists at high prevalence in wild Aedes vigilax populations in Sydney, Australia. Virology 2015; 486:272–283 [View Article]
    [Google Scholar]
  20. Harrison J, Warrilow D, McLean B, Watterson D, O’Brien C, O'Brien CA et al. A new Orbivirus isolated from mosquitoes in north-western Australia shows antigenic and genetic similarity to Corriparta virus but does not replicate in vertebrate cells. Viruses 2016; 8:141 [View Article]
    [Google Scholar]
  21. Colmant AMG, Bielefeldt-Ohmann H, Hobson-Peters J, Suen WW, O'Brien CA et al. A newly discovered flavivirus in the yellow fever virus group displays restricted replication in vertebrates. J Gen Virol 2016; 97:1087–1093 [View Article]
    [Google Scholar]
  22. Colmant AMG, Hobson-Peters J, Bielefeldt-Ohmann H, van den Hurk AF, Hall-Mendelin S et al. A new clade of insect-specific flaviviruses from Australian Anopheles mosquitoes displays species-specific host restriction. mSphere 2017; 2: [View Article]
    [Google Scholar]
  23. Prow NA, Mah MG, Deerain JM, Warrilow D, Colmant AMG et al. New genotypes of Liao ning virus (LNV) in Australia exhibit an insect-specific phenotype. Journal of General Virology 2018; 99:596–609 [View Article]
    [Google Scholar]
  24. Shi M, Neville P, Nicholson J, Eden J-S, Imrie A et al. High-resolution metatranscriptomics reveals the ecological dynamics of mosquito-associated RNA viruses in Western Australia. J Virol 2017; 91: [View Article]
    [Google Scholar]
  25. Prow NA, Tang B, Gardner J, Le TT, Taylor A et al. Lower temperatures reduce type I interferon activity and promote alphaviral arthritis. PLoS Pathog 2017; 13:e1006788 [View Article]
    [Google Scholar]
  26. Junglen S, Korries M, Grasse W, Wieseler J, Kopp A et al. Host range restriction of insect-specific flaviviruses occurs at several levels of the viral life cycle. mSphere 2017; 2: [View Article]
    [Google Scholar]
  27. Piyasena TBH, Setoh YX, Hobson-Peters J, Newton ND, Bielefeldt-Ohmann H et al. Infectious DNAs derived from insect-specific flavivirus genomes enable identification of pre- and post-entry host restrictions in vertebrate cells. Sci Rep 2017; 7:2940 [View Article]
    [Google Scholar]
  28. Boyd AM, Kay BH. Vector competence of Aedes aegypti, Culex sitiens, Culex annulirostris, and Culex quinquefasciatus (Diptera: Culicidae) for Barmah Forest virus. J Med Entomol 2000; 37:660–663 [View Article]
    [Google Scholar]
  29. Kay BH, Fanning ID, Carley JG. The vector competence of Australian Culex annulirostris with Murray Valley encephalitis and Kunjin viruses. Aust J Exp Biol Med 1984; 62:641–650 [View Article]
    [Google Scholar]
  30. Claflin SB, Webb CE. Ross River virus: many vectors and unusual hosts make for an unpredictable pathogen. PLoS Pathog 2015; 11:e1005070 [View Article]
    [Google Scholar]
  31. Jansen CC, Prow NA, Webb CE, Hall RA, Pyke AT et al. Arboviruses isolated from mosquitoes collected from urban and peri-urban areas of eastern Australia. J Am Mosq Control Assoc 2009; 25:272–278 [View Article]
    [Google Scholar]
  32. Reed LJ, Muench H. A simple method of estimating fifty per cent ENDPOINTS12. Am J Epidemiol 1938; 27:493–497 [View Article]
    [Google Scholar]
  33. Setoh YX, Prow NA, Peng N, Hugo LE, Devine G et al. De Novo Generation and Characterization of New Zika Virus Isolate Using Sequence Data from a Microcephaly Case. mSphere 2017; 2: [View Article]
    [Google Scholar]
  34. Hall RA, Burgess GW, Kay BH. Type-specific monoclonal antibodies produced to proteins of Murray Valley encephalitis virus. Immunol Cell Biol 1988; 66:51–56 [View Article]
    [Google Scholar]
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