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Volume 61, Issue 12

antifungal activity of the flavonoid baicalein against species Free

Abstract

The aim of the present study was to evaluate the activity of baicalein, the flavone constituent of , and synergism of the combination of baicalein and fluconazole against , and . The MIC (lowest concentration at which there was 50 % inhibition of growth) of baicalein alone against six strains ranged from 13 to 104 µg ml. For the three species tested, exposure to baicalein at the MIC concentrations obtained for each strain resulted in a high loss of viability. The fluconazole plus baicalein combination markedly reduced the MICs of both drugs for all three strains analysed. In addition, a synergistic effect between baicalein and fluconazole was observed for in terms of MIC (fractional inhibitory concentration index = 0.207). Scanning electron microscopy analysis revealed that yeast cells exposed to baicalein at MIC produced a profusely flocculent extracellular material, resembling a biofilm-like structure. In conclusion, these results showed the antifungal capability of baicalein against species and highlight a promising role of baicalein when used in combination with fluconazole against infections.

  • Received:
  • Accepted:
  • Published Online:
© 2012 SGM
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2012-12-01
2024-04-26
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References

  1. Al-Fattani M. A., Douglas L. J. 2006; Biofilm matrix of Candida albicans and Candida tropicalis: chemical composition and role in drug resistance. J Med Microbiol 55:999–1008 [View Article][PubMed]
     [Google Scholar]
  2. Arendrup M. C., Bruun B., Christensen J. J., Fuursted K., Johansen H. K., Kjaeldgaard P., Knudsen J. D., Kristensen L., Møller J. other authors 2011; National surveillance of fungemia in Denmark (2004 to 2009). J Clin Microbiol 49:325–334 [View Article][PubMed]
     [Google Scholar]
  3. Baillie G. S., Douglas L. J. 2000; Matrix polymers of Candida biofilms and their possible role in biofilm resistance to antifungal agents. J Antimicrob Chemother 46:397–403 [View Article][PubMed]
     [Google Scholar]
  4. Bruder-Nascimento A., Camargo C. H., Sugizaki M. F., Sadatsune T., Montelli A. C., Mondelli A. L., Bagagli E. 2010; Species distribution and susceptibility profile of Candida species in a Brazilian public tertiary hospital. BMC Res Notes 3:1–5 [View Article][PubMed]
     [Google Scholar]
  5. Cao Y. Y., Dai B. D., Wang Y., Huang S., Xu Y. G., Cao Y. B., Gao P. H., Zhu Z. Y., Jiang Y. Y. 2008; In vitro activity of baicalein against Candida albicans biofilms. Int J Antimicrob Agents 32:73–77 [View Article][PubMed]
     [Google Scholar]
  6. CLSI 2002 Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts 2nd edn; Approved Standard M27-A2 Wayne, PA: Clinical and Laboratory Standards Institute;
  7. Dai B.-D., Cao Y.-Y., Huang S., Xu Y.-G., Gao P.-H., Wang Y., Jiang Y.-Y. 2009; Baicalein induces programmed cell death in Candida albicans. J Microbiol Biotechnol 19:803–809[PubMed]
     [Google Scholar]
  8. Das I., Nightingale P., Patel M., Jumaa P. 2011; Epidemiology, clinical characteristics, and outcome of candidemia: experience in a tertiary referral center in the UK. Int J Infect Dis 15:e759–e763 [View Article][PubMed]
     [Google Scholar]
  9. Eliopoulos G. M., Moellering R. C. 1996; Antimicrobial combinations. In Antibiotics in Laboratory Medicine, 4th edn, pp. 330–396. Edited by V. Lorian. Baltimore: Williams & Wilkins..
    [Google Scholar]
  10. Favalessa O. C., Martins M. A., Hahn R. C. 2010; [Mycological aspects and susceptibility in vitro the yeast of the genus Candida from HIV-positive patients in the State of Mato Grosso]. Rev Soc Bras Med Trop 43:673–677 (in Portuguese) [View Article][PubMed]
     [Google Scholar]
  11. Fu Z., Lu H., Zhu Z. Y., Yan L., Jiang Y. Y., Cao Y. Y. 2011; Combination of baicalein and amphotericin B accelerates Candida albicans apoptosis. Biol Pharm Bull 34:214–218 [View Article][PubMed]
     [Google Scholar]
  12. Furlaneto M. C., Rota J. F., Quesada R. M. B., Furlaneto-Maia L., Rodrigues R., Oda S., Oliveira M. T., Serpa R., França E. J. G. 2011; Species distribution and in vitro fluconazole susceptibility of clinical Candida isolates in a Brazilian tertiary-care hospital over a 3-year period. Rev Soc Bras Med Trop 44:595–599 [View Article][PubMed]
     [Google Scholar]
  13. Hajjeh R. A., Sofair A. N., Harrison L. H., Lyon G. M., Arthington-Skaggs B. A., Mirza S. A., Phelan M., Morgan J., Lee-Yang W. other authors 2004; Incidence of bloodstream infections due to Candida species and in vitro susceptibilities of isolates collected from 1998 to 2000 in a population-based active surveillance program. J Clin Microbiol 42:1519–1527 [View Article][PubMed]
     [Google Scholar]
  14. Huang S., Cao Y. Y., Dai B. D., Sun X. R., Zhu Z. Y., Cao Y. B., Wang Y., Gao P. H., Jiang Y. Y. 2008; In vitro synergism of fluconazole and baicalein against clinical isolates of Candida albicans resistant to fluconazole. Biol Pharm Bull 31:2234–2236 [View Article][PubMed]
     [Google Scholar]
  15. Kang K., Fong W.-P., Tsang P. W.-K. 2010; Antifungal activity of baicalein against Candida krusei does not involve apoptosis. Mycopathologia 170:391–396 [View Article][PubMed]
     [Google Scholar]
  16. Kothavade R. J., Kura M. M., Valand A. G., Panthaki M. H. 2010; Candida tropicalis: its prevalence, pathogenicity and increasing resistance to fluconazole. J Med Microbiol 59:873–880 [View Article][PubMed]
     [Google Scholar]
  17. Lyon G. M., Karatela S., Sunay S., Adiri Y.Candida Surveillance Study Investigators 2010; Antifungal susceptibility testing of Candida isolates from the Candida surveillance study. J Clin Microbiol 48:1270–1275 [View Article][PubMed]
     [Google Scholar]
  18. McCourtie J., Douglas L. J. 1985; Extracellular polymer of Candida albicans: isolation, analysis and role in adhesion. J Gen Microbiol 131:495–503[PubMed]
     [Google Scholar]
  19. Mokaddas E. M., Al-Sweih N. A., Khan Z. U. 2007; Species distribution and antifungal susceptibility of Candida bloodstream isolates in Kuwait: a 10-year study. J Med Microbiol 56:255–259 [View Article][PubMed]
     [Google Scholar]
  20. Oxman D. A., Chow J. K., Frendl G., Hadley S., Hershkovitz S., Ireland P., McDermott L. A., Tsai K., Marty F. M. other authors 2010; Candidaemia associated with decreased in vitro fluconazole susceptibility: is Candida speciation predictive of the susceptibility pattern?. J Antimicrob Chemother 65:1460–1465 [View Article][PubMed]
     [Google Scholar]
  21. Pereira G. H., Müller P. R., Szeszs M. W., Levin A. S., Melhem M. S. C. 2010; Five-year evaluation of bloodstream yeast infections in a tertiary hospital: the predominance of non-C. albicans Candida species. Med Mycol 48:839–842 [View Article][PubMed]
     [Google Scholar]
  22. Pfaller M. A., Diekema D. J. 2010; Epidemiology of invasive mycoses in North America. Crit Rev Microbiol 36:1–53 [View Article][PubMed]
     [Google Scholar]
  23. Silva S., Negri M., Henriques M., Oliveira R., Williams D. W., Azeredo J. 2012; Candida glabrata, Candida parapsilosis and Candida tropicalis: biology, epidemiology, pathogenicity and antifungal resistance. FEMS Microbiol Rev 36:288–305 [View Article][PubMed]
     [Google Scholar]
  24. van Asbeck E. C., Clemons K. V., Stevens D. A. 2009; Candida parapsilosis: a review of its epidemiology, pathogenesis, clinical aspects, typing and antimicrobial susceptibility. Crit Rev Microbiol 35:283–309 [View Article][PubMed]
     [Google Scholar]
  25. Wong K. S., Tsang W. K. 2009; In vitro antifungal activity of the aqueous extract of Scutellaria baicalensis Georgi root against Candida albicans. Int J Antimicrob Agents 34:284–285 [View Article][PubMed]
     [Google Scholar]
  26. Yang Y.-L., Ho Y.-A., Cheng H.-H., Ho M., Lo H.-J. 2004; Susceptibilities of Candida species to amphotericin B and fluconazole: the emergence of fluconazole resistance in Candida tropicalis. Infect Control Hosp Epidemiol 25:60–64 [View Article][PubMed]
     [Google Scholar]
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In vitro antifungal activity of the flavonoid baicalein against Candida species
J. Med. Microbiol. 61, 1704 (2012); https://doi.org/10.1099/jmm.0.047852-0
/content/journal/jmm/10.1099/jmm.0.047852-0
/content/journal/jmm/10.1099/jmm.0.047852-0
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