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Volume 158, Issue 4

Roles of thioredoxin and thioredoxin reductase in the resistance to oxidative stress in Free

Abstract

The strain Shirota used in this study has in the genome four putative thioredoxin genes designated , , and , and one putative thioredoxin reductase gene designated . To elucidate the roles of the thioredoxins and the thioredoxin reductase against oxidative stress in , we constructed gene disruption mutants, in which each of the genes , and , or both and were disrupted, and we characterized their growth and response to oxidative stresses. In aerobic conditions, the (MS108) and the (MS109) mutants had moderate growth defects, and the double mutant (MS110) had a severe growth defect, which was characterized by elongation of doubling time and a lower final turbidity level. Furthermore, the mutant (MS111), which is defective in thioredoxin reductase, lost the ability to grow under aerobic conditions, although it grew partially under anaerobic conditions. The growth of these mutants, however, could be substantially restored by the addition of dithiothreitol or reduced glutathione. In addition, MS110 and MS111 were more sensitive to hydrogen peroxide and disulfide stress than the wild-type. In particular, the stress sensitivity of MS111 was significantly increased. On the other hand, transcription of all these genes was only weakly affected by these oxidative stresses. Taken together, these results suggest that the thioredoxin–thioredoxin reductase system is the major thiol/disulfide redox system and is essential to allow the facultative anaerobe to grow under aerobic conditions.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© 2012 SGM
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2012-04-01
2024-04-25
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Roles of thioredoxin and thioredoxin reductase in the resistance to oxidative stress in Lactobacillus casei
Microbiology 158, 953 (2012); https://doi.org/10.1099/mic.0.053942-0
/content/journal/micro/10.1099/mic.0.053942-0
/content/journal/micro/10.1099/mic.0.053942-0
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