Studies on the Transposable Element Tyl of Yeast
I. RNA Homologous to Tyl
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Excerpt
I. RNA HOMOLOGOUS TO Tyl
Tyl is a repeated, transposable DNA element in the genome of the yeast Saccharomyces cerevisiae (Cameron et al. 1979). Tyl has a structure (shown in Fig. 1) where the δs are direct terminal repeats of 338 bp (Gafner and Philippsen 1980). The entire element is repeated about 30 times in the haploid yeast genome. In this paper the RNA homologous to the Tyl DNA is characterized. There are two, large, abundant RNAs homologous to Tyl DNA, and the amount of the larger RNA varies in cells of different mating type.
Structure of Tyl RNA. RNA homologous to Tyl was identified by electrophoretically resolving total yeast nucleic acid followed by transfer to diazobenzyl-oxymethyl (DBM) paper (Wahl et al. 1979). The gel transfer was then hybridized with 32P-labeled pBR322-S13. The only yeast DNA present in this plasmid is a complete copy of the Tyl element (Cameron et...
