Template boundary definition in mammalian telomerase
Abstract
Telomerase uses a short template sequence in its intrinsic RNA component to synthesize telomere repeats. Disruption of the helix P1b in human telomerase RNA or alteration of its distance from the template resulted in telomerase copyingresidues past the normal template boundary both in vivo and in vitro. Therefore, helix P1b is important for template boundary definition in human telomerase. Mouse telomerase RNA lacks helix P1b, and the boundary is established at 2 nt downstream of the 5′-end. The divergent structure of boundary definition elements in mammals, yeast, and ciliates suggests diverse mechanisms for template boundary definition in telomerase.
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Footnotes
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Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.1140303.
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↵1 Corresponding author. E-MAIL cgreider{at}jhmi.edu; FAX (410) 614-2987.
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- Accepted September 16, 2003.
- Received August 4, 2003.
- Cold Spring Harbor Laboratory Press
