Human Y5 RNA specializes a Ro ribonucleoprotein for 5S ribosomal RNA quality control

J. Robert Hogg; Kathleen Collins

doi:10.1101/gad.1603907

Human Y5 RNA specializes a Ro ribonucleoprotein for 5S ribosomal RNA quality control

J. Robert Hogg1,3 and
Kathleen Collins2

Department of Molecular and Cell Biology, University of California at Berkely, Berkeley, California 94720 USA

Abstract

Humans express four distinct non-protein-coding Y RNAs (ncRNAs). To investigate Y RNA functional diversification, we exploited an RNA-based affinity purification method to isolate ribonucleoproteins (RNPs) assembled on individual human Y RNAs. Silver staining and mass spectrometry revealed that the Ro and La proteins assemble with all Y RNAs, while additional proteins associate with specific Y RNAs. Unexpectedly, Y5 RNA uniquely copurified ribosomal protein L5 and its binding partner 5S RNA. These findings reveal a contribution of Y5 to 5S surveillance and suggest that interactions between Ro-Y5 and L5–5S RNPs establish 5S RNA as a target of quality control.

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Footnotes

↵1 Present address: Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
↵2 Corresponding authors.

↵2 E-MAIL kcollins{at}berkeley.edu; FAX (510) 643-6334.
↵3 E-MAIL jh2721{at}columbia.edu; FAX .
Supplemental material is available at http://www.genesdev.org.
Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.1603907
- Received August 13, 2007.
- Accepted October 11, 2007.