Blue-light-dependent interaction of cryptochrome 1 with SPA1 defines a dynamic signaling mechanism
- Hong-Li Lian1,
- Sheng-Bo He1,
- Yan-Chun Zhang1,
- Dan-Meng Zhu2,
- Jing-Yi Zhang1,
- Kun-Peng Jia1,
- Shu-Xia Sun3,
- Ling Li1 and
- Hong-Quan Yang1,4
- 1Plant Science Institute, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China;
- 2Peking–Yale Joint Center of Plant Molecular Genetics and Agrobiotechnology, College of Life Sciences, Peking University, Beijing 100871, China;
- 3Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
Abstract
Cryptochromes (CRYs) are blue-light photoreceptors that mediate various light responses in plants and animals. The signaling mechanism by which CRYs regulate light responses involves their physical interactions with COP1. Here, we report that CRY1 interacts physically with SPA1 in a blue-light-dependent manner. SPA acts genetically downstream from CRYs to regulate light-controlled development. Blue-light activation of CRY1 attenuates the association of COP1 with SPA1 in both yeast and plant cells. These results indicate that the blue-light-triggered CRY1–SPA1 interaction may negatively regulate COP1, at least in part, by promoting the dissociation of COP1 from SPA1. This interaction and consequent dissociation define a dynamic photosensory signaling mechanism.
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Footnotes
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↵4 Corresponding author.
E-MAIL hqyang{at}sjtu.edu.cn; FAX (086) 21-3420-5877.
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Article published online ahead of print. Article and publication date are online at http://www.genesdev.org/cgi/doi/10.1101/gad.2025111.
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Supplemental material is available for this article.
- Received December 22, 2010.
- Accepted February 28, 2011.
- Copyright © 2011 by Cold Spring Harbor Laboratory Press
