The Prp19 complex is a novel transcription elongation factor required for TREX occupancy at transcribed genes

  1. Katja Sträßer1
  1. Gene Center Munich, Department of Biochemistry, Ludwig-Maximilians-University Munich, 81377 Munich, Germany, and Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-University Munich, 81377 Munich, Germany

    Abstract

    Different steps in gene expression are intimately linked. In Saccharomyces cerevisiae, the conserved TREX complex couples transcription to nuclear messenger RNA (mRNA) export. However, it is unknown how TREX is recruited to actively transcribed genes. Here, we show that the Prp19 splicing complex functions in transcription elongation. The Prp19 complex is recruited to transcribed genes, interacts with RNA polymerase II (RNAPII) and TREX, and is absolutely required for TREX occupancy at transcribed genes. Importantly, the Prp19 complex is necessary for full transcriptional activity. Taken together, we identify the Prp19 splicing complex as a novel transcription elongation factor that is essential for TREX occupancy at transcribed genes and that thus provides a novel link between transcription and messenger ribonucleoprotein (mRNP) formation.

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    Footnotes

    • 1 Corresponding author.

      E-MAIL strasser{at}lmb.uni-muenchen.de; FAX 49-89-218076945.

    • Article published online ahead of print. Article and publication date are online at http://www.genesdev.org/cgi/doi/10.1101/gad.623411.

    • Supplemental material is available for this article.

    • Received January 12, 2011.
    • Accepted March 30, 2011.
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