Phosphate-buffered saline (PBS)
| Reagent | Amount to add (for 1× solution) |
Final concentration (1×) | Amount to add (for 10× stock) | Final concentration (10×) |
|---|---|---|---|---|
| NaCl | 8 g | 137 mm | 80 g | 1.37 m |
| KCl | 0.2 g | 2.7 mm | 2 g | 27 mm |
| Na2HPO4 | 1.44 g | 10 mm | 14.4 g | 100 mm |
| KH2PO4 | 0.24 g | 1.8 mm | 2.4 g | 18 mm |
| If necessary, PBS may be supplemented with the following: | ||||
| CaCl2•2H2O | 0.133 g | 1 mm | 1.33 g | 10 mm |
| MgCl2•6H2O | 0.10 g | 0.5 mm | 1.0 g | 5 mm |
| PBS can be made as a 1× solution or as a 10× stock. To prepare 1 L of either 1× or 10× PBS, dissolve the reagents listed above in 800 mL of H2O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H2O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle or by filter sterilization. Store PBS at room temperature. | ||||
- © 2006 Cold Spring Harbor Laboratory Press
