黄曲霉毒素B1对雏鸡免疫器官影响的病理学观察

doi:10.11843/j.issn.0366-6964.2015.08.022

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (8): 1447-1454.doi: 10.11843/j.issn.0366-6964.2015.08.022

黄曲霉毒素B1对雏鸡免疫器官影响的病理学观察

于正强^1#，陈瑾^2#，彭西^1*，方静¹，陈科杰¹，何杨¹

（1.四川农业大学动物医学院，成都 611130；2.西昌市农牧局，西昌 615000）

收稿日期:2014-11-12 出版日期:2015-08-23 发布日期:2015-08-23
通讯作者: 彭西（1973-），教授，E-mail：pengxi197313@163.com
作者简介:于正强（1990-），男，重庆荣昌人，硕士，主要从事动物病理学研究，E-mail:mine_yzq@163.com；陈瑾（1988-），女，四川冕宁人，硕士，主要从事动物病理学研究， E-mail：chenjin19880808@126.com。于正强、陈瑾为并列第一作者
基金资助:
“教育部长江学者和创新团队发展计划”创新团队（IRT 0848）；四川省科技厅资助项目（2013FZ0072）

Effect of Aflatoxin B₁on Pathological Changes of Immune Organs in Broilers

YU Zheng-qiang^1# ，CHEN Jin^2# ，PENG Xi^1* ，FANG Jing¹，CHEN Ke-jie¹，HE Yang¹

（1.College of Veterinary Medicine，Sichuan Agricutural University，Chengdu 611130，China；2.Animal Husbandry Bureau of Xichang，Xichang 615000，China）

Received:2014-11-12 Online:2015-08-23 Published:2015-08-23

摘要/Abstract

摘要：

为探明黄曲霉毒素B1对雏鸡免疫器官组织学及超微结构的影响，将100只1日龄艾维茵健康公雏随机分为4组，分别喂以对照日粮和AFB1日粮（AFB₁Ⅰ、Ⅱ、Ⅲ组日粮中AFB1添加量分别为0.15、0.3和0.6 mg•kg^-1），试验期21 d。结果显示，AFB₁Ⅱ组和Ⅲ组雏鸡的免疫器官脏器指数显著下降（P<0.05）。AFB₁组雏鸡的免疫器官组织学损伤表现：胸腺皮质区网状细胞周围见较多细胞核碎片；法氏囊淋巴滤泡内细胞核碎片增多，滤泡髓质区淋巴细胞减少；脾白髓区细胞核碎片增多。超微病理学观察，胸腺、法氏囊和脾内淋巴细胞线粒体肿胀，以染色质边移为特征的凋亡细胞数目增多。结果表明，摄食含0.15～0.6 mg•kg^-1 AFB1的日粮，可不同程度地抑制雏鸡免疫器官的发育，致免疫器官中的淋巴细胞数量减少、细胞核碎片增多。

Abstract:

The aim of the current study was to investigate the histopathological and ultrastructural changes caused by dietary AFB₁ in broilers.One hundred one-day-old avian male broilers were randomly divided into four equal groups and were fed for 21 days as follows：a control diet and three AFB₁ addition diets containing 0.15，0.3 and 0.6 mg•kg^-1 AFB₁，respectively.The results showed that the relative weight of the three organs were lower than those of the control group (P<0.05).Histopathologically，in the AFB1 groups，there were increased nuclear debris around the reticulocytes in the cortex of the thymus；the number of lymphocytes was decreased in the medulla，and increased nuclear debris can be observed in the lymphoid follicle of the Bursa of Fabricius；more nuclear debris appeared around lymphoid follicles and lymphatic sheath in the chicken spleens.The ultrastructural changes were mitochondria swelling and increased apoptotic cells characterized as chromatin margination in the lymphocytes of the three immune organs.These results indicated that when the contents of dietary AFB₁were from 0.15 to 0.6 mg•kg^-1，the development of immune organs could be inhibited，and the major lesions of the three immune organs were the decrease of lymphocytes and the increase of nuclear debris.

中图分类号:

于正强，陈瑾，彭西，方静，陈科杰，何杨. 黄曲霉毒素B1对雏鸡免疫器官影响的病理学观察[J]. 畜牧兽医学报, 2015, 46(8): 1447-1454.

YU Zheng-qiang，CHEN Jin，PENG Xi，FANG Jing，CHEN Ke-jie，HE Yang. Effect of Aflatoxin B₁on Pathological Changes of Immune Organs in Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1447-1454.

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黄曲霉毒素B1对雏鸡免疫器官影响的病理学观察

Effect of Aflatoxin B₁on Pathological Changes of Immune Organs in Broilers

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黄曲霉毒素B1对雏鸡免疫器官影响的病理学观察

Effect of Aflatoxin B1 on Pathological Changes of Immune Organs in Broilers

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Effect of Aflatoxin B₁on Pathological Changes of Immune Organs in Broilers