Alterations Induced by Citrinin in Cultured Kidney Cells

Generoso Manoel Chagas; Maria Lúcia Wambier Klüppel; Annibal de Paiva Campello; Dorly de Freitas Buchi; Maria Benigna Martinelli de Oliveira

doi:10.1247/csf.19.103

Generoso Manoel Chagas, Maria Lúcia Wambier Klüppel, Annibal de Paiva Campello, Dorly de Freitas Buchi, Maria Benigna Martinelli de Oliveira

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Keywords: citrinin, mycotoxin, BHK-21 cells, electron microscopy, transplasma membrane redox systems

JOURNAL FREE ACCESS

1994 Volume 19 Issue 2 Pages 103-108

DOI https://doi.org/10.1247/csf.19.103

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Abstract

The cytotoxicity of citrinin was evaluated in an established cell line of baby hamster kidney cells. The primary effect of the mycotoxin was on the adherence of the cells to the culture bottles. Microscopic evaluation of morphological alterations indicated that the cells which were originally elongated and flattened, became swollen and round. Electron microscopic examination showed that citrinin (0.1, 0.5 and 1.0mM) incubated for 10 hours with cultured cells, promoted drastic alterations of normal mitochondria, with swelling and cell death. Transplasma membrane redox system is inhibited by citrinin (81%). This effect is dependent not only on the toxin concentration, but also on the time of exposure to the cells.

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