Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Isolation and Characterization of a New Abortifacient Protein, Karasurin, from Root Tubers of Trichosanthes kirilowii MAX. var. japonicum KITAM.
Shunsuke TOYOKAWATadahiro TAKEDAYukio OGIHARA
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1991 Volume 39 Issue 3 Pages 716-719

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Abstract

A new abortifacient protein, named karasurin, was isolated from fresh root tubers of Trichosanthes kirilowii MAXIMOWICZ var. japonicum KITAMURA (Cucurbitaceae, Japanese name : kikarasuuri) by the procedure involving acetone fractionation and ion-exchange chromatography on Toyopearlpak SP 650S. Homogeneity of Karasurin was demonstrated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, and high performance liquid chromatography (HPLC). Karasurin was a highly basic protein of pI 10.1 and the molecular weight was estimated as 28000 by SDS-polyacrylamide gel electrophoresis. Karasurin showed a strong abortion effect in pregnant mice.

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© The Pharmaceutical Society of Japan
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