Sulfoquinovosyldiacylglycerol, KM043, a New Potent Inhibitor of Eukaryotic DNA Polymerases and HIV-Reverse Transcriptase Type 1 from a Marine Red Alga, Gigartina tenella

Keisuke OHTA; Yoshiyuki MIZUSHIMA; Noriko HIRATA; Masaharu TAKEMURA; Fumio SUGAWARA; Akio MATSUKAGE; Shonen YOSHIDA; Kengo SAKAGUCHI

doi:10.1248/cpb.46.684

Keisuke OHTA, Yoshiyuki MIZUSHIMA, Noriko HIRATA, Masaharu TAKEMURA, Fumio SUGAWARA, Akio MATSUKAGE, Shonen YOSHIDA, Kengo SAKAGUCHI

Author information

Keisuke OHTA
Yoshiyuki MIZUSHIMA
Noriko HIRATA
Masaharu TAKEMURA
Fumio SUGAWARA
Akio MATSUKAGE
Shonen YOSHIDA
Kengo SAKAGUCHI
Author's Organization：
Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo:Development, Toyo Suisan Kaisya, LTd.,
Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo
Development, Toyo Suisan Kaisha, Ltd.,
Laboratory of Cancer Cell Biology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine
Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo
Laboratory of Cell Biology, Aichi Cancer Center Research Institute
Laboratory of Cancer Cell Biology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine
Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo

Keywords: DNA polymerase, Gigartina tenella, inhibitor, sulfoquinovosyldiacylglycerol (SQDG)

JOURNAL FREE ACCESS

1998 Volume 46 Issue 4 Pages 684-686

DOI https://doi.org/10.1248/cpb.46.684

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Abstract

A new sulfolipid, KM043, which belongs to the 6-sulfo-α-D-quinovopyranosyl-(1→3')-1', 2'-diacylglycerol (SQDG) class of compounds, has been isolated from a marine red alga, Gigartina tenella, as a potent inhibitor of eukaryotic DNA polymerases and HIV-reverse transcriptase type 1. Its structure was determined on the basis of spectroscopic and gas chromatographic analyses. The inhibition was dose-dependent, and complete (more than 90%)inhibition of DNA polymerase α (pol. α), DNA polymerase β (pol. β) and HIV-reverse transcriptase type 1 (HIV-RT) was observed at concentrations of 5, 10, and 30 μM, respectively.

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