Two new sterols, (22E)-23-methylergosta-5, 7-22-trien-3β-ol (1) and 5α, 6α-epoxy-(22E)-ergosta-8, 14, 22-triene-3β, 7α-diol (2), have been isolated from two edible mushrooms, Lentinula edodes and Tricholoma matsutake, respectively, together with twelve known ones (3-14). The structures of the new compounds were elucidated on the basis of their spectral data.