Chemical and Pharmaceutical Bulletin
Online ISSN : 1347-5223
Print ISSN : 0009-2363
ISSN-L : 0009-2363
Regular Articles
Isolation and Structure Determination of Bioactive Isoflavones from Callus Culture of Dipteryx odorata
Ana Helena Januário, Miriam Verginia Lourenço, Lilian Aparecida Domézio, Rosemeire Cristina Linhari Rodrigues Pietro, Marcelo Santos Castilho, Daniela Maria Tomazela, Maria Fátima das Graças Fernandes da Silva, Paulo Cezar Vieira, João Batista Fernandes, Suzelei de Castro França
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Keywords: Dipteryx odorata, isoflavone, callus culture, GAPDH inhibitor
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2005 Volume 53 Issue 7 Pages 740-742

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Abstract

Crude extracts of callus and roots of Dipteryx odorata were analyzed by HPLC to detect and quantify isoflavone contents. Based on spectroscopic and X-ray crystallography data the structures of two isoflavones were elucidated as 7-hydroxy-4′,6-dimethoxyisoflavone and 3′,7-dihydroxy-4′,6-dimethoxyisoflavone. The production of dry biomass of 7-hydroxy-4′,6-dimethoxyisoflavone in cultured callus was 4.12 mg/g, approximately eleven fold higher than the amount accumulated in roots of D. odorata wild-growing plants. The 7-hydroxy-4′,6-dimethoxyisoflavone was effective against glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi. This is the first report on those bioactive isoflavones accumulated in callus of D. odorata.

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© 2005 The Pharmaceutical Society of Japan
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