Two new neolignan glucosides, bidenlignasides A (1) and B (2), were isolated from the whole plant of Bidens parviflora WILLD. The structures of the two new compounds 1 and 2 established to be 3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-[5E-(3-hydroxypropenyl)-3-methoxy-2-O-β-D-glucosyl)phenyl] propan-1-one and 3-{3-[1,3-dihydroxy-3-(4-hydroxy-3-methoxyphenyl)propyl]-4-hydroxy-5-methoxyphenyl}-allyl-O-β-D-glucuside, respectively, on the basis of spectral and chemical evidence. Compounds 1 and 2 were found to inhibit histamine release from peritoneal exudate mast cells induced by antigen-antibody reaction.