Twenty years of ribosome assembly and ribosomopathies
- Department of Cell Biology, Albert Einstein College of Medicine, New York, New York 10461, USA
- Corresponding author: jon.warner{at}einstein.yu.edu
This extract was created in the absence of an abstract.
During the 20 years of RNA extraordinary progress has been made in the study of ribosomes, beyond that of ribosome structure, which will be well covered by others.
Ribosome assembly
One can argue that in 1968 Nomura and colleagues set back the field of ribosome assembly by showing that a bacterial ribosome could be assembled from its constituent rRNA and ribosomal protein (RP) molecules with no help from any catalytic factors. Yet shortly thereafter we found that HeLa pre-rRNA was complexed with numerous proteins that were not incorporated into the final ribosome, and suggested that many catalytically acting factors were involved in ribosome assembly. Only decades later did the techniques of yeast genetics and of TAP purification/Mass Spectrometry allow the labs of Woolford, Baserga, Tschochner, and many others to identify the catalytic molecules involved, and to show that they are necessary for the production of ribosomes. During the past 20 years it …
