The Effects of Acute Exposure to Deoxynivalenol on Some Inflammatory Parameters in Miniature Pigs

Osamu MIKAMI; Midori KUBO; Hideo MURATA; Yoshihiro MUNETA; Yasuyuki NAKAJIMA; Shigeru MIYAZAKI; Nobuhiko TANIMURA; Ken KATSUDA

doi:10.1292/jvms.10-0461

Clinical Pathology

The Effects of Acute Exposure to Deoxynivalenol on Some Inflammatory Parameters in Miniature Pigs

Osamu MIKAMI ^†, Midori KUBO ^†, Hideo MURATA ^†, Yoshihiro MUNETA ^†, Yasuyuki NAKAJIMA ^†, Shigeru MIYAZAKI ^†, Nobuhiko TANIMURA ^†, Ken KATSUDA ^†

Author information

Osamu MIKAMI ^†
Safety Research Team, National Institute of Animal Health
Research Team for Environmental/Enzootic Diseases, Tohoku Research Station, National Institute of Animal Health
Midori KUBO ^†
Safety Research Team, National Institute of Animal Health
Kamikawa Livestock Hygiene Service Center
Hideo MURATA ^†
Safety Research Team, National Institute of Animal Health
Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University
Yoshihiro MUNETA ^†
Research Team for Advanced Biologicals, National Institute of Animal Health
Yasuyuki NAKAJIMA ^†
Safety Research Team, National Institute of Animal Health
Shigeru MIYAZAKI ^†
Safety Research Team, National Institute of Animal Health
Hokkaido Research Station, National Institute of Animal Health
Nobuhiko TANIMURA ^†
Safety Research Team, National Institute of Animal Health
Ken KATSUDA ^†
Research Team for Environmental/Enzootic Diseases, Tohoku Research Station, National Institute of Animal Health
Author contributions

†These authors contributed equally: Osamu MIKAMI, Midori KUBO, Hideo MURATA, Yoshihiro MUNETA, Yasuyuki NAKAJIMA, Shigeru MIYAZAKI, Nobuhiko TANIMURA, Ken KATSUDA

Keywords: acute-phase protein, cytokine, deoxynivalenol, neutrophil, swine

JOURNAL FREE ACCESS

2011 Volume 73 Issue 5 Pages 665-671

DOI https://doi.org/10.1292/jvms.10-0461

View "Advance Publication" version (January 6, 2011).

Details

Abstract

Seven miniature pigs were injected intravenously with deoxynivalenol (DON) at 1 mg/kg body weight; afterward, the number of leukocytes in peripheral blood, the luminol-dependent chemiluminescence of neutrophils, the serum or plasma concentration of cytokines and acute-phase proteins were evaluated to determine the effects of acute exposure to DON on inflammatory responses. White blood cell counts were transiently increased at 3, 6, and 12 hr post-injection (PI) due to the increased number of neutrophils. The luminol-dependent chemiluminescence value of neutrophils was significantly elevated at 24 hr PI, indicating the activation of the bactericidal function of neutrophils. Significant increases of interleukin (IL)-8 and tumor necrosis factor-α at 3 hr PI and IL-6 at 6 hr PI were detected in the serum. The concentration of haptoglobin and serum amyloid A was significantly increased at 24 hr PI. These results suggest that acute exposure to DON induced a temporary recruitment of neutrophils in the peripheral blood by IL-8 and subsequent activation of the bactericidal function, and a transient increase of proinflammatory cytokines and acute-phase proteins, indicating the immunomodulatory effects of DON in pigs.

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