Large-Scale Survey of Mitochondrial D-Loop of the Red-Crowned Crane Grus japonensis in Hokkaido, Japan by Convenient Genotyping Method

Yoshiaki MIURA; Akira SHIOMI; Junya SHIRAISHI; Kohei MAKITA; Mitsuhiko ASAKAWA; Takio KITAZAWA; Takeo HIRAGA; Yulia MOMOSE; Kunikazu MOMOSE; Hiroyuki MASATOMI; Hiroki TERAOKA

doi:10.1292/jvms.12-0193

Biochemistry

Large-Scale Survey of Mitochondrial D-Loop of the Red-Crowned Crane Grus japonensis in Hokkaido, Japan by Convenient Genotyping Method

Yoshiaki MIURA, Akira SHIOMI, Junya SHIRAISHI, Kohei MAKITA, Mitsuhiko ASAKAWA, Takio KITAZAWA, Takeo HIRAGA, Yulia MOMOSE, Kunikazu MOMOSE, Hiroyuki MASATOMI, Hiroki TERAOKA

Author information

Yoshiaki MIURA
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan
Akira SHIOMI
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan
Junya SHIRAISHI
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan
Kohei MAKITA
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan
Mitsuhiko ASAKAWA
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan
Takio KITAZAWA
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan
Takeo HIRAGA
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan
Yulia MOMOSE
NPO Tancho Protection Group, Kushiro 085–0036, Japan
Kunikazu MOMOSE
NPO Tancho Protection Group, Kushiro 085–0036, Japan
Hiroyuki MASATOMI
NPO Tancho Protection Group, Kushiro 085–0036, Japan
Hiroki TERAOKA
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan
NPO Tancho Protection Group, Kushiro 085–0036, Japan

Keywords: Grus japonensis, Hokkaido, Japan, mitochondria, Red-crowned Crane

JOURNAL FREE ACCESS

2013 Volume 75 Issue 1 Pages 43-47

DOI https://doi.org/10.1292/jvms.12-0193

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Abstract

The Red-crowned Crane, Grus japonensis, is an endangered species of crane that has two separate breeding populations, one in the Amur River basin (continental population) and the other in eastern or northern Hokkaido, Japan (island population). So far, only two haplotypes (Gj1 and Gj2) have been identified in the mitochondrial D-loop in island population, whereas seven haplotypes have been found in continental population (Gj3–Gj9). We developed a rapid and inexpensive method of extensive genotyping of D-loop haplotypes in Red-crowned Cranes, based on amplification refractory mutation system (ARMS) PCR assay. Two hundred and three cranes in eastern Hokkaido were studied with this method and supplemental DNA sequencing. Only two haplotypes, Gj1 and Gj2, were confirmed in eastern Hokkaido with Gj2 as a major haplotype. Additionally, only Gj2 was identified in twelve feathers from both sexes found in northern Hokkaido. These results suggest scarce genetic diversity in island population of Red-crowned Cranes in Hokkaido, Japan.

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