Czech J. Food Sci., 2011, 29(2):181-189 | DOI: 10.17221/300/2010-CJFS

Antioxidant capacity and antioxidants of strawberry, blackberry, and raspberry leaves

Lucie Buřičová1, Mirjana Andjelkovic2, Anna Čermáková1, Zuzana Réblová1, Ondřej Jurček3,4,5, Erkki Kolehmainen4, Roland Verhé2, František Kvasnička6
1 Department of Food Chemistry and Analysis, 3Department of Chemistry of Natural Compounds and 6Department of Food Preservation and Meat Technology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic
2 Department of Organic Chemistry, Ghent University, Ghent, Belgium; 4Department of Chemistry, Laboratory of Organic Chemistry, University of Jyväskylä, Jyväskylä, Finland
5 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Isotope Laboratory, Prague, Czech Republic

The total phenolic content (Folin-Ciocalteu method), free radical scavenging ability expressed as DPPH value, ferric reducing antioxidant capacity (FRAP), and oxygen radical absorbance capacity (ORAC) were determined in water extracts of leaves from Rosaceae family plants (Fragaria vesca L., Rubus fructicosus L., and Rubus idaeus L.). The antioxidant capacities of the extracts (in the order of the above mentioned methods) were 73.6-88.9%, 60.1-71.4%, 49.7-78.0% respectively, and 45.3-66.5% of that of green tea water extract. Further, the presence of 15 compounds (gallic acid, rutin, ellagic acid, caffeic acid, p-coumaric acid, quercetin, kaempferol, myricetin, quercetin-3-d-glucoside, ascorbic acid, (+)-catechin, (-)-epicatechin, epicatechingallate, epigallocatechin, procyanidin B1) was studied by HPLC-ECD and their antioxidant capacities were compared to the antioxidant capacity of the extracts. Out of the compounds studied, mostly (+)-catechin, ellagic acid, and (-)-epicatechin participated in the antioxidant capacities of the studied plant leaves water extracts. The antioxidant capacity of leaves infusions (determined by DPPH method) was lower than those of red wines and tea infusions, but comparable to the antioxidant capacities of white wines and fruit beverages.

Keywords: DPPH; Folin-Ciocalteau method; Fragaria vesca; Rubus fructicosus; Rubus ideaus; FRAP; HPLC; ORAC

Published: April 30, 2011  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Buřičová L, Andjelkovic M, Čermáková A, Réblová Z, Jurček O, Kolehmainen E, et al.. Antioxidant capacity and antioxidants of strawberry, blackberry, and raspberry leaves. Czech J. Food Sci.. 2011;29(2):181-189. doi: 10.17221/300/2010-CJFS.
Download citation

References

  1. Benzie I.F.F., Strain J.J. (1996): The ferric reduction ability of plasma (FRAP) as measurement of "antioxidant power": The Frap assay. Analytical Biochemistry, 239: 70-76. Go to original source... Go to PubMed...
  2. Buřičová L., Réblová Z. (2008): Czech medicinal plants as possible sources of antioxidants. Czech Journal and Food Sciences, 26: 132-138. Go to original source...
  3. Capecka E., Mareczek A., Leja M. (2005): Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food Chemistry, 93: 223-226. Go to original source...
  4. Dorman H.J., Bachmayer O., Kosar M., Hiltunen R. (2004): Antioxidant properties of aqueous extracts from selected Lamiaceae species grown in Turkey. Journal of Agricultural and Food Chemistry, 52: 762-770. Go to original source... Go to PubMed...
  5. Farhoosh R., Golmovahhed G.A., Khodaparast M.H.H. (2007): Antioxidant activity of various extracts of old tea leaves and black tea wastes (Camellia sinensis L.). Food Chemistry, 100: 231-236. Go to original source...
  6. Gadow A., Joubert E., Hansmann CH.F. (1997): Comparison of the antioxidant activity of aspalathin with that of other plant phenols of rooibos tea (Aspalathus linearis), α-tocopherol, BHT, and BHA. Journal of Agricultural and Food Chemistry, 45: 632-638. Go to original source...
  7. Gardner E.J., Ruxton C.H.S., Leeds A.R. (2007): Black tea - helpful or harmful? A review of the evidence. European Journal of Clinical Nutrition, 61: 3-18. Go to original source... Go to PubMed...
  8. Gudej J., Rychlínska I. (1996): Flavonoid compounds from the leaves of Rubus ideaus L. Herba Polonica, 4: 257-261.
  9. Gudej J. (2003): Kaempferol and quercetin glycosides from Rubus ideaus L. leaves. Acta Poloniae Pharmaceutica, 60: 313-316.
  10. Gudej J., Tomczyk M. (2004): Determination of flavonoids, tannins, ellagic acid in leaves from Rubus L. species. Archives of Pharmacal Research, 27: 1114-1119. Go to original source... Go to PubMed...
  11. Halliwell B. (2006): Polyphenols: Antioxidant treats for healthy living or covert toxins. Journal of the Science of Food and Agriculture, 86:1992-1995. Go to original source...
  12. Hanhineva K., Soininen P., Anttonen M.J., Kokko H., Rogachev I., Aharoni A., Laatikainen R., Kärenlampi S. (2009): NMR and UPLC-qTOF-MS/MS characterisation of novel phenylethanol derivatives of phenylpropanoid glucosides from the leaves of strawberry (Fragaria × ananassa cv. Jonsok). Phytochemical Analysis, 20: 353-364. Go to original source... Go to PubMed...
  13. Huang D., Ou B., Hampsch-Woodill M., Flanagan J.A., Prior R.L. (2002): High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. Journal of Agricultural and Food Chemistry, 50: 4437-4444. Go to original source... Go to PubMed...
  14. Huang D., Ou B., Prior R.L. (2005): The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry, 53: 1841-1856. Go to original source... Go to PubMed...
  15. Hukkanen A.T., Kokko H.I., Buchala A.J., McDougall G.J., Stewart D., Kärenlampi S.O., Karjalainen R.O. (2007): Benzothiadiazole induces the accumulation of phenolics and improves resistance to powdery mildew in strawberries. Journal of Agricultural and Food Chemistry, 55: 1862-1870. Go to original source... Go to PubMed...
  16. Katalinic V., Milos M., Modun D., Music I., Boban M. (2004): Antioxidant effectiveness of selected wines in comparation with (+)-catechin. Food Chemistry, 86: 593-600. Go to original source...
  17. Katalinic V., Milos M., Kulisic T., Jukic M. (2006): Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemistry, 94: 550-557. Go to original source...
  18. Konfik M., Papagiannopoulos M., Galensa R. (2007): Enantioseparation of catechin and epicatechin in plant food by chiral capillary electrophoresis. European Food Research and Technology, 225: 569-577. Go to original source...
  19. Kris-Etherton P.M., Hecker K.D., Bonanome A., Coval S.M., Binkoski A.E., Hilpert K.F., Griel A.E., Etherton T.D. (2002): Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. American Journal of Medicine, 133: 71-88. Go to original source... Go to PubMed...
  20. Lin Y.L., Juan I.M., Chen Y.L., Liang Y.CH., Lin J.K. (1996): Composition of polyphenols in fresh tea leaves and associations of their oxygen-radical-absorbing capacity with antiproliferative actions in fibroblast cells. Journal of Agricultural and Food Chemistry, 44: 1387-1394. Go to original source...
  21. Logan B.A., Hammond M.P., Stormo B.M. (2008): The French paradox: determining the superoxide-scavening capacity of red wine and other beverages. Biochemistry and Molecular Biology Education, 36: 39-42. Go to original source... Go to PubMed...
  22. Lange D.W. de, Verhoef S., Gorter G., Kraaijenhagen R.J., van de Wiel A., Akkerman J.W.N. (2007): Polyphenolic grape extract inhibits platalet activation through PECAM-1: An explanation for the French paradox. Alcoholism: Clinical and Experimental Research, 31: 1308-1314. Go to original source... Go to PubMed...
  23. Mendoza-Wilson A.M., Glossman-Mitnik D. (2006): Theoretical study of the molecular properties and chemical reactivity of (C)-catechin and (K)-epicatechin related to their antioxidant ability. Journal of Molecular Structure (Teochem), 761: 97-106. Go to original source...
  24. Mudnic I., Modun D., Brizic I., Vukovic J., Generalic I., Katalinic V., Bilusic T., Ljubenkov I., Boban M. (2009): Cardiovascular effects in vitro of aqueous extract of wild strawberry (Fragaria vesca L.) leaves. Phytomedicine, 16: 462-469. Go to original source... Go to PubMed...
  25. Murakami M., Yamaguchi T., Takamura H., Matoba T. (2003): Effects of ascorbic acid and alpha-tocopherol on antioxidant activity of polyphenolic compounds. Journal of Food Sciences, 8: 1622-1625. Go to original source...
  26. Pellegrini N., Salvatore S., Valtuena S., Bedogni G., Porrini M., Pala V., Del Rio D., Sieri S., Miglio C., Krogh V., Zavaroni I., Brighenti F. (2007): Development and validation of a food frequency questionnaire for the assessment of dietary total antioxidant capacity. Journal of Nutrition, 137: 93-98. Go to original source... Go to PubMed...
  27. Priyadarsini I.K., Khopde S.M., Kumar S.S., Mohan H.J. (2002): Free radical studies of ellagic acid, a natural phenolic antioxidant. Journal of Agricultural and Food Chemistry, 50: 2200-2206. Go to original source... Go to PubMed...
  28. Roginsky V., Lissi E.A. (2005): Review of methods to determine chain-breaking antioxidant activity in food. Food Chemistry, 92: 235-254. Go to original source...
  29. Singleton V.L., Orthofer R., Lamuela-Raventos R.M. (1999): Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Oxidants and antioxidants Part A. Methods in Enzymology, 299: 152-178. Go to original source...
  30. Skupien K., Oszmianski J. (2004): Comparison of six cultivars of strawberries (Fragaria × ananassa Duch.) grown in northwest Poland. European Food Research and Technology, 219: 66-70. Go to original source...
  31. Svilaas A., Sakhi A.K., Andersen L.F., Svilaas T., Ström E.C., Jacobs D.R., Ose L., Blomhoff R. (2004): Intakes of antioxidants in coffee, wine and vegetables are correlated with plasma carotenoids in humans. Journal of Nutrition, 134: 562-567. Go to original source... Go to PubMed...
  32. Venskutonis P.R., Dvaranauskaite A., Labokas J. (2007): Radical scavenging activity and composition of raspberry (Rubus idaeus) leaves from different locations in Lithuania. Fitoterapia, 78: 162-165. Go to original source... Go to PubMed...
  33. Wang S.Y. (1999): Methyl jasmonate reduces water stress in strawberry. Journal of Plant Growth Regulation, 18: 127-134. Go to original source... Go to PubMed...
  34. Wang S.Y., Lin H.S. (2000): Antioxidant activity in fruits and leaves of blackberry, raspberry and strawberry varies with cultivar and developmental stage. Journal of Agricultural and Food Chemistry, 48: 140-146. Go to original source... Go to PubMed...
  35. Williamson G., Manach C. (2005): Bioavailability and bioefficacy of polyphenols in humans. II. Review of 93 intervention studies. American Journal of Clinical Nutrition, 81: 243S-255S. Go to original source... Go to PubMed...

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.