Vet Med - Czech, 2009, 54(3):118-124 | DOI: 10.17221/3087-VETMED

Expression of substance P, vasoactive intestinal peptide and galanin in cultured myenteric neurons from the ovine abomasums

M.B. Arciszewski1, S. Barabasz1, J. Calka2
1 Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland
2 Faculty of Veterinary Medicine, Warmia and Mazury University, Olsztyn, Poland

Enteric neurons are able to alter their neurotransmitter content during adaptation to new artificial conditions. The aim of the present study was to investigate changes in vasoactive intestinal peptide (VIP), substance P (SP) and galanin expression during culture of myenteric neurons from the ovine abomasum. In order to accurately reflect the in vivo situation cryostat sections from the ovine abomasum were used. Cultured and non-cultured myenteric neurons were immunohistochemically stained with a mixture of antibodies raised against Hu C/D (neuronal marker) and VIP, SP or galanin. Double labeling revealed that Hu C/D-IR/VIP-IR myenteric neurons were very rare in cryostat sections (1.4 ± 0.2%) but significantly increased to 21.3 ± 1.7% when cultured for three days. A significant increase in Hu C/D-positive/VIP-positive myenteric neurons were also found in 6- and 9-days cultures (23.9 ± 1.9 and 24.5 ± 2.0%, respectively). In vivo, the expression of SP was found in 9.7 ± 1.0% of myenteric perikarya. After 3, 6 and 9 days of incubation the proportion of Hu C/D-IR/SP-IR myenteric perikarya significantly increased to 19.3 ± 1.3%, 22.3 ± 1.2% and 24.1 ± 1.7% (respectively). When compared to the in vivo situation the proportion of galanin-expressing myenteric neurons was unchanged after 3, 6 and 9 days of culturing. In conclusion, alterations in VIP and SP (but not galanin) expression occur during neuronal culturing. Our results supports the idea that both VIP and SP may act as factors which increase neuronal survival.

Keywords: enteric nervous system; myenteric neurons; neuronal plasticity; neuropeptides; abomasum; sheep

Published: March 31, 2009  Show citation

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Arciszewski MB, Barabasz S, Calka J. Expression of substance P, vasoactive intestinal peptide and galanin in cultured myenteric neurons from the ovine abomasums. Vet Med-Czech. 2009;54(3):118-124. doi: 10.17221/3087-VETMED.
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References

  1. Arciszewski M.B., Ekblad E. (2005): Effects of vasoactive intestinal peptide and galanin on survival of cultured porcine myenteric neurons. Regulatory Peptides, 125, 185-192. Go to original source... Go to PubMed...
  2. Arciszewski M.B., Pierzynowski S., Ekblad E. (2005): Lipopolysaccharide induces cell death in cultured porcine myenteric neurons. Digestive Diseases and Sciences, 50, 1661-1668. Go to original source... Go to PubMed...
  3. Arciszewski M.B., Sand E., Ekblad E. (2008): Vasoactive intestinal peptide rescues cultured rat myenteric neurons from lipopolysaccharide induced cell death. Regulatory Peptides, 146, 218-223. Go to original source... Go to PubMed...
  4. Azzolina A., Bongiovanni A., Lampiasi N. (2003): Substance P induces TNF-α and IL-6 production through NFκB in peritoneal mast cells. Biochememica et Biophysica Acta, Molecular Cell Research, 1643, 75-83. Go to original source... Go to PubMed...
  5. Brenneman D.E., Hill J.M., Glazner G.W., Gozes I., Phillips T.M. (1995): Interleukin-1α and vasoactive intestinal peptide: enigmatic regulation of neuronal survival. International Journal of Developmental Neuroscience, 13, 187-200. Go to original source... Go to PubMed...
  6. Brenneman D.E., Phillips T.M., Hauser J., Hill J.M., Spong C.Y., Gozes I. (2003): Complex array of cytokines released by vasoactive intestinal peptide. Neuropeptides, 37, 111-119. Go to original source... Go to PubMed...
  7. Calvo N., Reiriz J., Perez-Navarro E., Alberch J. (1996): Tachykinins protect cholinergic neurons from quinolinic acid excitotoxicity in striatal cultures. Brain Research, 740, 323-328. Go to original source... Go to PubMed...
  8. Delgado M., Ganea D. (2003): Vasoactive intestinal peptide prevents activated microglia-induced neurodegeneration under inflammatory conditions: potential therapeutic role in brain trauma. FASEB Journal, 17, 1922-1924. Go to original source... Go to PubMed...
  9. Ekblad E., Bauer A.J. (2004): Role of vasoactive intestinal peptide and inflammatory mediators in enteric neuronal plasticity. Neurogastroenterololgy and Motility, 16 (Suppl. 1), 123-128. Go to original source... Go to PubMed...
  10. Ekblad E., Mulder H., Subdler F. (1996): Vasoactive intestinal peptide expression in enteric neurons is upregulated by both colchicines and axotomy. Regulatory Peptides, 63, 113-121. Go to original source...
  11. Ekblad E., Mei Q., Subdler F. (2000): Innervation of gastric mucosa. Microscopy Research and Technique, 48, 241-257. Go to original source... Go to PubMed...
  12. El-Salhy M. (1999): Neuroendocrine peptides in stomach and colon of an animal model for human diabetes type I. Journal of Diabetes and its Complications, 13, 170-173. Go to original source... Go to PubMed...
  13. Ferrante F., Geppetti P., Amenta F. (1991): Age-related changes in substance P and vasoactive intestinal polypeptide immunoreactivity in the rat stomach and small intestine. Archives of Gerontology and Geriatrics, 13, 81-87. Go to original source... Go to PubMed...
  14. Grider J.R., Bonilla O.M. (1994): Differential expression of substance P, somatostatin, and VIP in neurons from cultured myenteric ganglia. American Journal of Physiology, Gastrointestinal and Liver Physiology, 267, G322-G327. Go to original source... Go to PubMed...
  15. Hannibal J., Ekblad E., Mulder H., Sundler F., Fahrenkrug J. (1998): Pituitary adenylate cyclase activating polypeptide (PACAP) in the gastrointestinal tract of the rat: distribution and effects of capsaicin or denervation. Cell and Tissue Research, 291, 65-79. Go to original source... Go to PubMed...
  16. Ho W., Lai J., Zhu X., Uvaydova M., Douglas S.D. (1997): Human monocytes and macrophages express substance P, neurokinin-1 receptor. Journal of Immunology, 159, 5654-5660. Go to original source... Go to PubMed...
  17. Hu H.-Z., Gao N., Lin Z., Gao C., Liu S., Ren J., Xia Y., Wood J.D. (2002): Chemical coding and electrophysiology of enteric neurons expressing neurofilament 145 in guinea pig gastrointestinal tract. Journal of Comparative Neurology, 442, 189-203. Go to original source... Go to PubMed...
  18. Jarvinen M.K., Wollmann W.J., Powrozek T.A., Schultz J.A., Powley T.L. (1999): Nitric oxide synthase-containing neurons in the myenteric plexus of the rat gastrointestinal tract: distribution and regional density. Anatomy and Embryology, 199, 99-112. Go to original source... Go to PubMed...
  19. Klimaschewski L., Grohmann I., Heym C. (1996): Targetdependent plasticity of galanin and vasoactive intestinal peptide in the rat superior cervical ganglion after nerve lesion and reinnervation. Neuroscience, 72, 265-272. Go to original source... Go to PubMed...
  20. Lallemend F., Lefebvre P.P., Hans G., Rigo J.M., Van de Water T.R., Moonen G., Malgrange B. (2003): Substance P protects spiral ganglion neurons from apoptosis via PKC-Ca2+-MAPK/ERK pathways. Journal of Neurochemistry, 87, 508-521. Go to original source... Go to PubMed...
  21. Landry M., Holmberg K., Zhang X., Hokfelt T. (2000): Effect of axotomy on expression of NPY, galanin, and NPY Y1 and Y2 receptors in dorsal root ganglia and the superior cervical ganglion studied with double-labeling in situ hybridization and immunohistochemistry. Experimental Neurology, 162, 361-384. Go to original source... Go to PubMed...
  22. Lin Z., Sandgren K., Ekblad E. (2003): Increased expression of vasoactive intestinal polypeptide in cultured myenteric neurons from adult rat small intestine. Autonomic Neuroscience, 107, 9-19. Go to original source... Go to PubMed...
  23. Lotz M., Vaughan J.H., Carson D.A. (1988): Effect of neuropeptides on production of inflammatory cytokines by human monocytes. Science, 241, 1218-1221. Go to original source... Go to PubMed...
  24. McCormack R.J., Hart P.P., Ganea D. (1996): Expression of NK-1 receptor mRNA in murine T lymphocytes. Neuroimmunomodulation, 3, 35-46. Go to original source... Go to PubMed...
  25. Miyamoto Y., Yoneda M., Morikawa A., Itoh H., Makino I. (2001): Gastric neuropeptides and gastric motor abnormality in streptozotocin-induced diabetic rats. Digestive Diseases and Sciences, 46, 1596-1603. Go to original source... Go to PubMed...
  26. Neunlist M., Aubert P., Toquet C., Oreshkova T., Barouk J., Lehur P.A., Schemann M., Galmiche J.P. (2003): Changes in chemical coding of myenteric neurones in ulcerative colitis. Gut, 52, 84-90. Go to original source... Go to PubMed...
  27. Okada T., Hirayama Y., Kishi S., Miyayasu K., Hiroi J., Fujii T. (1999): Functional neurokinin NK-1 receptor expression in rat peritoneal mast cells. Inflammation Research, 48, 274-279. Go to original source... Go to PubMed...
  28. Pfannkuche H., Schellhorn C., Schemann M., Gabel G. (2003): Reticular groove and reticulum are innervated by myenteric neurons with different neurochemical codes. Anatomical Record Part A, 274A, 917-922. Go to original source... Go to PubMed...
  29. Pimont S., Bruley Des Varannes S., Le Neel J.C., Aubert P., Galmiche J.P., Neunlist M. (2003): Neurochemical coding of myenteric neurones in the human gastric fundus. Neurogastroenterology and Motility, 15, 655-662. Go to original source... Go to PubMed...
  30. Reiche D., Schemann M. (1999): Mucosa of the guinea pig gastric corpus is innervated by myenteric neurones with specific neurochemical coding and projection preferences. Journal of Comparative Neurology, 410, 489-502. Go to original source... Go to PubMed...
  31. Salthun-Lassalle B., Traver S., Hirsch E.C., Michel P.P. (2005): Substance P, neurokinins A and B and synthetic tachykinin peptides protect mesencephalic dopaminergic neurons in culture via an activity-dependent mechanism. Molecular Pharmacology, 68, 1214-1224. Go to original source... Go to PubMed...
  32. Sandgren K., Lin Z., Fex-Svenningsen A., Ekblad E. (2003): Vasoactive intestinal peptide and nitric oxide promote survival of adult rat myenteric neurons in culture. Journal of Neuroscience Research, 72, 595-602. Go to original source... Go to PubMed...
  33. Schicho R., Holzer P., Th. Lippe I. (2004): Gastric enteric neurones that respond to luminal injury. Neurogastroenterology and Motility, 16 (Suppl. 1), 129-132. Go to original source... Go to PubMed...
  34. Shochina M., Belai A., Toole L., Knight G., Burnstock G. (1997): Neurochemical coding in the myenteric plexus of the upper gastrointestinal tract of hibernating hamsters. International Journal of Developmental Neuroscience, 15, 353-362. Go to original source... Go to PubMed...
  35. Vanden Berghe P., Coulie B., Tack J., Mawe G.M., Schemann M., Janssens J. (1999): Neurochemical coding of myenteric neurons in the guinea-pig antrum. Cell and Tissue Research, 297, 81-90. Go to original source... Go to PubMed...
  36. Wiesenfeld-Hallin Z., Xu X.J. (2001): Neuropeptides in neuropathic and inflammatory pain with special emphasis on cholecystokinin and galanin. European Journal of Pharmacology, 429, 49-59. Go to original source... Go to PubMed...

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