Plant Soil Environ., 2014, 60(5):210-215 | DOI: 10.17221/73/2014-PSE

Application of chlorophyll fluorescence performance indices to assess the wheat photosynthetic functions influenced by nitrogen deficiencyOriginal Paper

M. Živčák1, K. Olšovská1, P. Slamka2, J. Galambošová3, V. Rataj3, H.B. Shao4, M. Brestič1
1 Department of Plant Physiology, Slovak University of Agriculture, Nitra, Slovak Republic
2 Department of Agrochemistry and Plant Nutrition, Slovak University of Agriculture, Nitra, Slovak Republic
3 Department of Machines and Production Systems, Slovak University of Agriculture, Nitra, Slovak Republic
4 Key Laboratory of Coastal Biology and Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai, P.R. China

Nitrogen deficiency influences importantly the plant photosynthetic capacity and crop productivity. Here, we employed the rapid, non-invasive measurements of chlorophyll a fluorescence kinetics for calculation of the integrative fluorescence parameters related to the leaf photosynthetic performance. In pot experiments with winter wheat (Triticum aestivum L.) we cultivated plants during the whole growing period in the soil substrate supplied with four different doses of nitrogen. The leaf nitrogen and chlorophyll content as well as the plant dry mass were analyzed after chlorophyll fluorescence records in three growth stages. Our results indicate that the commonly used parameter Fv/Fm (the maximum quantum yield of photochemistry) was almost insensitive to nitrogen treatment. In contrary, the performance index (PIabs) and total performance index (PItot) were much more responsive and significant differences among plants of different nitrogen treatments as well as between the youngest and third leaf from the top were observed. Parameter PItot was shown to express only small diurnal changes, thus being more reliable and more useful for comparison of different samples in field conditions than more frequently used parameter PIabs.

Keywords: performance index; photosynthesis; plant ecophysiology; non-invasive techniques; JIP-test

Published: May 31, 2014  Show citation

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Živčák M, Olšovská K, Slamka P, Galambošová J, Rataj V, Shao HB, Brestič M. Application of chlorophyll fluorescence performance indices to assess the wheat photosynthetic functions influenced by nitrogen deficiency. Plant Soil Environ.. 2014;60(5):210-215. doi: 10.17221/73/2014-PSE.
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    References

    1. Antal T., Mattila H., Hakala-Yatkin M., Tyystjärvi T., Tyystjärvi E. (2010): Acclimation of photosynthesis to nitrogen deficiency in Phaseolus vulgaris. Planta, 232: 887-898. Go to original source... Go to PubMed...
    2. Brestič M., Živčák M., Kalaji H.M., Carpentier R., Allakhverdiev S.I. (2012): Photosystem II thermostability in situ: Environmentally induced acclimation and genotype-specific reactions in Triticum aestivum L. Plant Physiology and Biochemistry, 57: 93-105. Go to original source... Go to PubMed...
    3. Brestič M., Živčák M. (2013): PSII fluorescence techniques for measurement of drought and high temperature stress signal in crop plants: Protocols and applications. In: Rout G.R., Das A.B. (eds.): Molecular Stress Physiology of Plants. SpringerVerlag, Berlin, Heidelberg. Go to original source...
    4. Guidi L., Lorefice G., Pardossi A., Malorgio F., Tognoni F., Soldatini G.F. (1997): Growth and photosynthesis of Lycopersicon esculentum (L.) plants as affected by nitrogen deficiency. Biologia Plantarum, 40: 235-244. Go to original source...
    5. Janušauskaite D., Feiziene D. (2012): Chlorophyll fluorescence characteristics throughout spring triticale development stages as affected by fertilization. Acta Agriculturae Scandinavica, Section B - Soil and Plant Science, 62: 7-15. Go to original source...
    6. Kalaji H.M., Carpentier R., Allakhverdiev S.I., Bosa K. (2012): Fluorescence parameters as early indicators of light stress in barley. Journal of Photochemistry and Photobiology B: Biology, 112: 1-6. Go to original source... Go to PubMed...
    7. Li G., Zhang Z.S., Gao H.Y., Liu P., Dong S.T., Zhang J.W., Zhao B. (2012): Effects of nitrogen on photosynthetic characteristics of leaves from two different stay-green corn (Zea mays L.) varieties at the grain-filling stage. Canadian Journal of Plant Science, 92: 671-680. Go to original source...
    8. Lichtenthaler H.K. (1987): Chlorophyll and carotenoids - Pigments of photosynthetic biomembranes. Methods in Enzymology, 148: 350-382. Go to original source...
    9. Lu C., Zhang J., Zhang Q., Li L., Kuang T. (2001): Modification of photosystem II photochemistry in nitrogen deficient maize and wheat plants. Journal of Plant Physiology, 158: 1423-1430. Go to original source...
    10. Nikiforou C., Manetas Y. (2011): Inherent nitrogen deficiency in Pistacia lentiscus preferentially affects photosystem I: A seasonal field study. Functional Plant Biology, 38: 848-855. Go to original source... Go to PubMed...
    11. Redillas M.C.F.R., Jeong J.S., Strasser R.J., Kim Y.S., Kim J.K. (2011): JIP analysis on rice (Oryza sativa cv. Nipponbare) grown under limited nitrogen conditions. Journal of the Korean Society for Applied Biological Chemistry, 54: 827-832. Go to original source...
    12. Ripley B.S., Redfern S.P., Dames J.F. (2004): Quantification of the photosynthetic performance of phosphorus-deficient Sorghum by means of chlorophyll-a fluorescence kinetics. South African Journal of Science, 100: 615-618.
    13. Schansker G., Tóth S.Z., Strasser R.J. (2005): Methylviologen and dibromothymoquinone treatments of pea leaves reveal the role of photosystem I in the Chl a fluorescence rise OJIP. Biochimica et Biophysica Acta, 1706: 250-261. Go to original source... Go to PubMed...
    14. Smit M.F., van Heerden P.D., Pienaar J.J., Weissflog L., Strasser R.J., Krüger G.H. (2009): Effect of trifluoroacetate, a persistent degradation product of fluorinated hydrocarbons, on Phaseolus vulgaris and Zea mays. Plant Physiology and Biochemistry, 47: 623-634. Go to original source... Go to PubMed...
    15. Stirbet A., Govindjee (2011): On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and photosystem II: Basics and applications of the OJIP fluorescence transient. Journal of Photochemistry and Photobiology B, 104: 236-257. Go to original source... Go to PubMed...
    16. Strasser B.J., Strasser R.J. (1995): Measuring fast fluorescence transients to address environmental questions: The JIP-test. In: Mathis P. (ed.): Photosynthesis: from Light to Biosphere. Kluwer Academic Publishers, Dordrecht. Go to original source...
    17. Strasser R.J., Srivastava A., Tsimilli-Michael M. (2000): The Fluorescence Transient as a Tool to Characterize and Screen Photosynthetic Samples. Probing Photosynthesis: Mechanisms, Regulation and Adaptation. Taylor and Francis, New York, London.
    18. Terashima I., Evans J.R. (1988): Effects of light and nitrogen nutrition on the organization of the photosynthetic apparatus in spinach. Plant and Cell Physiology, 29: 143-155.
    19. Van Heerden P.D., Strasser R.J., Krüger G.H. (2004): Reduction of dark chilling stress in N-fixing soybean by nitrate as indicated by chlorophyll a fluorescence kinetics. Physiologia Plantarum, 121: 239-249. Go to original source... Go to PubMed...
    20. Van Heerden P.D., Tsimilli-Michael M., Krüger G.H., Strasser R.J. (2003): Dark chilling effects on soybean genotypes during vegetative development: Parallel studies of CO 2 assimilation, chlorophyll a fluorescence kinetics O-J-I-P and nitrogen fixation. Physiologia Plantarum, 117: 476-491. Go to original source... Go to PubMed...
    21. Vassileva V., Demirevska K., Simova-Stoilova L., Petrova T., Tsenov N., Feller U. (2012): Long-term field drought affects leaf protein pattern and chloroplast ultrastructure of winter wheat in a cultivar-specific manner. Journal of Agronomy and Crop Science, 198: 104-117. Go to original source...
    22. Živčák M., Brestič M., Olšovská K. (2008a): Physiological parameters useful in screening for improved tolerance to drought in winter wheat (Triticum aestivum L.). Cereal Research Communication, 36: 1943-1946.
    23. Živčák M., Brestič M., Olšovská K, Slamka P. (2008b): Performance index as a sensitive indicator of water stress in Triticum aestivum L. Plant, Soil and Environment, 54: 133-139. Go to original source...
    24. Živčák M., Brestič M., Kalaji H.M., Govindjee (2014): Photosynthetic responses of sun- and shade-grown barley leaves to high light: Is the lower PSII connectivity in shade leaves associated with protection against excess of light? Photosynthesis Research, 119: 339-354. Go to original source... Go to PubMed...

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