Archives of Biological Sciences 2012 Volume 64, Issue 1, Pages: 71-76
https://doi.org/10.2298/ABS1201071L
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Chemical compositions, A-glucosidase and A-amylase inhibitory activities of crude polysaccharides from the endodermis of shaddock (Citrus maxima)
Liu Guocong (Yulin Normal University, Yulin, Guangxi, PR China)
The chemical composition of shaddock mainly includes polyphenols, proteins
and polysaccharides. However, polysaccharides from shaddock materials have
received much less consideration than polyphenols (Fellers et al., 1990).
Herein the chemical compositions, α-glucosidase and α-amylase inhibitory
activities of crude polysaccharides from the endodermis of shaddock were
investigated. The exopolysaccharides (EPS) exhibited a broad and intense peak
at 3300-3400 cm-1 that characterized the absorption of the hydroxyl group,
and one weak C-H band at around 2941.3 cm-1 in the IR spectrum. The content
of neutral sugars in EPS was determined as 37.16%. The content of acidic
sugar in EPS was determined as 33.71%. EPS exhibited the highest content of
neutral sugar. The content of proteins in EPS was 5.75%. The content of
polyphenols was 6.52%. The EPS mainly consisted of four types of
polysaccharides with molecular weights of 110 kD, 68 kD, 31 kD and 12 kD. The
crude EPS showed significantly higher inhibitory effects on α-glucosidase and
α-amylase (inhibition to 74.12% and 86.59%, respectively).
Keywords: Endodermis of shaddock, crude polysaccharides, chemical compositions, α-glucosidase and α-amylase inhibitory activities