doiSerbia

Protective effect of probiotic bacteria against cadmium-induced genotoxicity in rat hepatocytes in vivo and in vitro

Jama Adel M. (Faculty of Biology, Chair of Comparative Physiology and Ecophysiology, Belgrade)
Mitić-Ćulafić Dragana (Faculty of Biology, Chair of Microbiology, Belgrade)
Kolarević S. (Faculty of Biology, Chair of Microbiology, Belgrade)
Đurašević Siniša F. (Faculty of Biology, Chair of Comparative Physiology and Ecophysiology, Belgrade)
Knežević-Vukčević Jelena (Faculty of Biology, Chair of Microbiology, Belgrade)

The protective effect of probiotic bacteria against cadmium (Cd)-induced genotoxicity was studied in rat hepatocytes in vivo and in vitro. Male Wistar rats, Rattus norvegicus, were treated for five weeks with (i) CdCl2 (70 ppm in the drinking water), (ii) a mixture of lyophilized probiotic bacteria Lactobacillus rhamnosus, L. acidophilus and Bifido-bacterium longum (5×108 cfu/g of food), or (iii) CdCl2 and probiotic bacteria. In addition, single cells obtained from the untreated rat liver were exposed to CdCl2 (70 ppm), probiotic bacteria (1.28 mg/ml), or CdCl2 and probiotic bacteria, for 15 min at 22°C in the dark. The level of Cd-induced DNA damage in hepatocytes was determined by the comet assay. The obtained results show that probiotic bacteria significantly reduced Cd-induced genotoxicity, both in vivo and in vitro (20% and 48%, respectively). Moreover, the toxicity of Cd to lactobacilli in the gastrointestinal tracts of rats was significantly decreased in the probiotic-treated animals. The binding of Cd2+ to probiotic bacteria was proposed as the most probable protection mechanism.

Keywords: Cadmium, toxicity, lactic acid bacteria, genotoxicity, rat hepatocytes

Acknowledgments. This research was financially supported by the Ministry of Education and Science of the Government of Serbia, projects No 172058 and 173023