Trends in analytical methods used in mycotoxin analyses over the last 25 years are discussed based on results from WHO and FAPAS^®. In 1978, most determinations of aflatoxin B₁ in maize samples were done utilizing TLC methods. The percentage of TLC methods decreased to 48 % in 1989 and to 7 % in 2002. Alternatively, the use of HPLC has increased from less than 5 % in 1978 to 36 % in 1989 and 77 % in 2002. ELISA based methods have also been used in more recent tests, but the percentage of laboratories using ELISA is not high. The percentage of data falling within |z| ≤ 2 increased steadily from 50 % in 1978 to 82 % in 2002. Similar trends over time were observed for aflatoxin B₁ in peanut, aflatoxin M₁ in milk and ochratoxin A data.