Isolation and Identification of Myricitrin, an Antioxidant Flavonoid, from Daebong Persimmon Peel

In-Wook Hwang; Shin-Kyo Chung

doi:10.3746/pnf.2018.23.4.341

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저널정보

한국식품영양과학회
Preventive Nutrition and Food Science 학술저널
Preventive Nutrition and Food Science Vol.23 No.4
2018.12 341 - 346 (6page)
DOI : 10.3746/pnf.2018.23.4.341

저자정보

In-Wook Hwang (Dong-A University)
Shin-Kyo Chung (Kyungpook National University)

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초록·키워드

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In this study, the antioxidant flavonoid, myricitrin, was isolated and identified from Daebong persimmon peel. The persimmon peel extract was successively fractionated with n-hexane, ethyl acetate, and n-butanol. The ethyl acetate fraction had the strongest antioxidant activities among the solvent fractions and was further fractionated by silica gel and octadecylsilane column chromatography, and preparative high performance liquid chromatography. Three antioxidant compounds were finally isolated, and compound 2 was identified as myricitrin by liquid chromatography/mass spectrometry and ¹H and ¹³C nuclear magnetic resonance. Myricitrin had the strongest antioxidant activities by ferric ion reducing antioxidant power and α,α-diphenyl-2-picrylhydrazyl radical scavenging assays. These results suggested that myricitrin was found as a major antioxidant flavonoid responsible for the strong antioxidant activities of Daebong persimmon peels.

#antioxidant #myricitrin #persimmon peel #LC/MS #NMR