Binding properties of rat prorenin and renin to the recombinant rat renin/prorenin receptor prepared by a baculovirus expression system

  • Authors:
    • A.H.M. Nurun Nabi
    • Ayano Kageshima
    • Mohammad Nasir Uddin
    • Tsutomu Nakagawa
    • Enoch Y. Park
    • Fumiaki Suzuki
  • View Affiliations

  • Published online on: September 1, 2006     https://doi.org/10.3892/ijmm.18.3.483
  • Pages: 483-488
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Abstract

The rat recombinant renin/prorenin receptor (AB188298 in DDJB), which conjugated with FLAG epitope in its N-terminus, was expressed in a baculovirus expression system. The recombinant receptor, prepared from the cytoplasmic fraction of the insect cells, was identified by Western blotting using anti-FLAG antibody. Prorenin as well as renin bound to the receptor with different binding affinities. Their Kd values were estimated at 8.0 and 20 nM, respectively. The amounts of prorenin and renin bound to the immobilized receptors were 1.0 and 0.2 pmole, respectively. The prorenin bound to the receptor had renin activity and the renin kept the activity at similar level to that before the binding. The Km of their complexes was the same at 3.3 µM when sheep angiotensinogen was used as the substrate. Their Vmax values were 5.5 and 10 nM·h−1, respectively. The molecular activities of prorenin and renin bound to the receptor were 1.1 and 10 h−1, respectively. From these findings, rat prorenin as well as renin was indicated to bind to the recombinant receptor and express the enzymatic activity in vitro.

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September 2006
Volume 18 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Nabi AN, Kageshima A, Uddin MN, Nakagawa T, Park EY and Suzuki F: Binding properties of rat prorenin and renin to the recombinant rat renin/prorenin receptor prepared by a baculovirus expression system. Int J Mol Med 18: 483-488, 2006
APA
Nabi, A.N., Kageshima, A., Uddin, M.N., Nakagawa, T., Park, E.Y., & Suzuki, F. (2006). Binding properties of rat prorenin and renin to the recombinant rat renin/prorenin receptor prepared by a baculovirus expression system. International Journal of Molecular Medicine, 18, 483-488. https://doi.org/10.3892/ijmm.18.3.483
MLA
Nabi, A. N., Kageshima, A., Uddin, M. N., Nakagawa, T., Park, E. Y., Suzuki, F."Binding properties of rat prorenin and renin to the recombinant rat renin/prorenin receptor prepared by a baculovirus expression system". International Journal of Molecular Medicine 18.3 (2006): 483-488.
Chicago
Nabi, A. N., Kageshima, A., Uddin, M. N., Nakagawa, T., Park, E. Y., Suzuki, F."Binding properties of rat prorenin and renin to the recombinant rat renin/prorenin receptor prepared by a baculovirus expression system". International Journal of Molecular Medicine 18, no. 3 (2006): 483-488. https://doi.org/10.3892/ijmm.18.3.483