A Renewable C Reactive Protein Amperometric Immunosensor Based on Magnetic Multiwalled Carbon Nanotubes Probles Modified Electrode

Shao Lin Zeng; Han Kun Zhou; Ning Gan; Yu Ting Cao

doi:10.4028/www.scientific.net/AMM.80-81.452

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 80-81A Renewable C Reactive Protein Amperometric...

A Renewable C Reactive Protein Amperometric Immunosensor Based on Magnetic Multiwalled Carbon Nanotubes Probles Modified Electrode

Abstract:

A disposable, magnetism-controlled, and amperometric immunosensor was fabricated for the determination of C reactive protein (CRP). Firstly, Fe₃O₄ (core)/Au (shell) nanoparticle (GMP)–coated multiwalled carbon nanotubes (MWCNT–GMP) were prepared. Then, monoclonal antibody (anti-CRP) was immobilized on MWCNT–GMP to prepare MWCNT–GMP/anti-CRP composite magnetic probes. At last, the probes was adsorbed on the surface of N,N''-bis-(2-hydroxy-methylene)-o-phenylenediamine cobalt (CoRb) modified, screen-printed carbon electrodes through external magnetic field.The amount of CRP in the serum sample was determined by one-step immunoassay. When different concentrations of CRP were added into25μL of phosphate-buffered solution (pH7.0) containing10^-4M H₂O₂, the percentage of DPV cathodic peak current decrease (CR%) exhibited a linear relationship with the concentration of CRP in the range of 0.3–100 μg L^-1.The detection limit was 0.16 μg L^-1(3б).The immunosensor was used to determine CRP in serum samples of patients with heart diseases, and the results were consistent with those of the traditional ELISA method. The proposed amperometric immunosensor was sensitive, rapid, magnetic field–controlled, and disposable; therefore, it could be used to determine even traces of CRP in the blood serums of patients with heart diseases.

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Periodical:

Applied Mechanics and Materials (Volumes 80-81)

Pages:

452-456

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.80-81.452

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Online since:

July 2011

Authors:

Shao Lin Zeng, Han Kun Zhou, Ning Gan, Yu Ting Cao

Keywords:

CRP, Magnetic Carbon Nanotube Composite Particles, Magnetic Enzyme-Free Immunosensor, N,N'-Bis-(2-Hydroxy-Methylene)-O Phenylenediamine Cobalt, Renewable Electrode

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