Open Access, Peer-reviewed
pISSN 2288-3886
eISSN 2288-3959
    Korean J Nutr. 2010 Aug;43(4):342-350. Korean.
    Published online Aug 31, 2010.
    https://doi.org/10.4163/kjn.2010.43.4.342
    © 2010 The Korean Nutrition Society
    Original Article

    Effects of Soy Protein, its Hydrolysate and Peptide Fraction on Lipid Metabolism and Appetite-Related Hormones in Rats

    Ji-Hye Park,1 Mi-Na Park,1 Im-Sik Lee,2 Yong-Ki Kim,2 Wan-Sik Kim,2 and Yeon-Sook Lee1
      • 1Department of Food and Nutrition & Research Institute of Human Ecology, Seoul National University, Seoul 151-742, Korea.
      • 2R & D Center, Maeil Dairies Co. Ltd., Pyungtaek 451-861, Korea.
    • To whom correspondence should be addressed. (Email: lysook@snu.ac.kr )
    Received May 20, 2010; Revised June 20, 2010; Accepted August 04, 2010.

    Abstract

    This study was aimed to investigate whether soy protein hydrolysates had beneficial effects on serum and tissue lipid contents and appetite-related hormones as compared with intact soy protein. Four-week-old male Sprague-Dawley rats were fed AIN-93M diet containing high fat (18% w/w) with low protein (10% w/w). After four weeks, the rats were divided into four groups (n = 8/group) and fed experimental diets with different nitrogen sources and levels, respectively; 10% soy protein isolate (10SPI), 25% soy protein isolate (25SPI), 25% soy protein hydrolysates (25SPH) and 25% soy macro-peptide fractions (25SPP, MW ≥ 10,000) for six weeks. Weight gain was significantly higher in 25% nitrogen sources-fed groups than in 10% group (10SPI). In 25SPP, perirenal fat mass and serum total lipid were significantly lower than in other groups. As for appetite-related hormones, serum ghrelin concentration was not shown to be different among groups but leptin concentration was significantly decreased in 25SPP. It can be concluded that soy macro-peptide fractions as compared with intact soy protein may have beneficial effects on reducing fat mass and serum lipid.

    Keywords
    soy protein; hydrolysate; macro-peptide fraction; lipid; appetite

    Figures

    Fig. 1
    The concentration of serum glucose and insulin in rats fed high fat diets containing various nitrogen sources. Groups are the same as in Table 1. Values are means ± SE of 8 rats per group. Bars with different superscripts are significantly different at p < 0.05 by Duncan's multiple range test.

    Fig. 2
    The concentration of serum ghrelin and leptin in rats fed high fat diets containing various nitrogen sources. Groups are the same as in Table 1. Values are means ± SE of 8 rats per group. Bars with different superscripts are significantly different at p < 0.05 by Duncan's multiple range test.

    Tables

    Table 1
    Composition of experimental diets (g/kg diet)1)

    Table 2
    Body weight, weight gain and food intake of the rats fed high fat containing various nitrogen sources

    Table 3
    Fresh weights of liver, kidney, testis and spleen in rats fed high fat diets containing various nitrogen sources (g/100 g BW)

    Table 4
    Fresh weight of perirenal and epididymal fat in rats fed high fat containing various nitrogen sources

    Table 5
    The concentration of serum total lipid, triglyceride, total cholesterol and HDL cholesterol in rats fed high fat containing various nitrogen sources (mg/dL)

    Table 6
    The contents of liver total lipid, triglyceride and total cholesterol in rats fed high fat containing various nitrogen sources (mg/g)

    Table 7
    Fecal excretion of total lipid, triglyceride and total cholesterol in rats fed high fat containing various nitrogen sources

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    MeSH Terms
    Animals
    Appetite
    Diet
    Ghrelin
    Humans
    Leptin
    Lipid Metabolism
    Male
    Nitrogen
    Rats
    Rats, Sprague-Dawley
    Soybean Proteins
    Weight Gain
    Metrics
    Share
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