Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2009, 153(2):103-110 | DOI: 10.5507/bp.2009.017

SALIVA AS A DIAGNOSTIC MEDIUM

Richard Pinka, Jiri Simeka, Jana Vondrakovab, Edgar Faberb, Petr Michla, Jindrich Pazderaa, Karel Indrakb
a Department of Oral and Maxillofacial Surgery, University Hospital, Olomouc, 775 20, Czech Republic,
b Department of Hemato-Oncology, University Hospital, Olomouc, 775 20 Czech Republic

Background: This is a review of current knowledge on the use of saliva, gingival cervical fluid and mucosal transudate in the detection of some oral and systemic diseases as well as drugs. Oral fluid is a diagnostic medium that can be easily collected and with minimal invasion but it has been neglected in the past. Today, saliva is being used more often to diagnose: HIV virus, oro-facial and systemic tumors, cardiovascular disease and in detecting addictive substances. Neutropil levels in saliva may also indicate successful bone marrow transplant. Oral fluid is now systematically being researched and oral fluid analysis is being compared with the analysis of other diagnostic media such as blood and urine . A number of recent studies have focused on oncogenic marker detection and its monitoring in saliva. The latest clinical and laboratory findings on diagnostic markers of oropharyngeal carcinoma in oral fluid could be the beginning of their wider use as a diagnostic medium. Oral fluid can also be also used to diagnose other malignancies such as breast cancer which was one of the first malignant tumors to be detected using genetic protein biomarkers. Raised levels of CA15-3 and the epidermal growth factor (EGF) receptor have been found in patients with breast cancer and elevated levels of CA 125 and the glycoprotein complex in the saliva of ovarian cancer patients.

Conclusion: Doubtless, the diagnostic value of saliva, aided by current technological development will increase rapidly in the near future.

Keywords: Keywords: Saliva, Biomarkers, Diagnostics

Received: March 23, 2009; Accepted: May 7, 2009; Published: June 1, 2009  Show citation

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Pink, R., Simek, J., Vondrakova, J., Faber, E., Michl, P., Pazdera, J., & Indrak, K. (2009). SALIVA AS A DIAGNOSTIC MEDIUM. Biomedical papers, 153(2), 103-110. doi: 10.5507/bp.2009.017
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    References

    1. de Almeida Pdel V, Gregio AM, Machado MA, de Lima AA, Azevedo LR. Saliva composition and functions: a comprehensive review. J Contemp Dent pract. 2008; 9:72-80. Go to original source...
    2. Turner RJ. Mechanisms of fluid secretion by salivary glands. Ann. NY Acad. Sci 1993; 694:24-35. Go to original source... Go to PubMed...
    3. Kassan SS, Moutsopoulos HM.Clinical manifestation and early diagnosis of Sjögrens syndrome. Arch. Intern. Med. 2004; 164: 1275-84. Go to original source... Go to PubMed...
    4. Kassan SS, Thomas TL, Moutsopoulos HM, et al. Increased risk of lymphoma in sicca syndrome. Arch. Intern. med. 1978; 89: 888-92 Go to original source... Go to PubMed...
    5. Theander E, Henriksson G, Ljungberg O, Mandl T, Manthorpe R, Jacobsson LT. Lymphoma and other malignancies in primary Sjogrens syndrome: a cohort study on cancer incidence and lymphoma predictors. Ann. Rheum. Dis 2006; 65:796-803. Go to original source... Go to PubMed...
    6. Vitali C, Bombardieri S, Jonsson R. The European Study Group on classification criteria for Sjögrens syndrome: a revised version of the AmericanEuropean Consensus Group. Ann. Rheum. Dis 2002; 61: 554-8. Go to original source... Go to PubMed...
    7. Daniels TE. Labial salivary gland biopsy in Sjögrens syndrome: assessment as a diagnostic tool criterion in 362 suspected cases. Arthritis Rheum 1984; 27: 147-56. Go to original source... Go to PubMed...
    8. Ryu OH, Atkinson JC, Hoehn GT, Illei GG, Hart TC Identification of parotid salivary biomarkers in Sjögrens syndrome by surface-enhanced laser desorption ionization-time-of-flight-mass spectrometry and two-dimensional difference gel electrophoresis. Rheumatology (Oxford) 2006; 45: 1077-86. Go to original source... Go to PubMed...
    9. Boyle JO, Mao L, Brennan JA, et al. Gene mutations in saliva as molecular markers for head and neck squamous cell carcinomas. Am J Surg 1994; 168: 429-32. Go to original source... Go to PubMed...
    10. Warnakulasuriya S, Soussi T, Maher R, Johnson N, Tavassoli M Expression of p53 in oral squamous cell carcinoma is associated with the presence of IgG and IgA p53 autoantibodies in sera and saliva of the patients. J Pathol 2002; 192: 52-7. Go to original source... Go to PubMed...
    11. Zhao M, Rosenbaum E, Carvalho AL, et al. Feasibility of quantitative PCR-based saliva rince screening of HPV for head and neck cancer. Int J Cancer 2005; 117: 605-10. Go to original source... Go to PubMed...
    12. Smith EM, Ritchie JM, Summersgill KF, et al. Human papillomavirus in oral exfoliated cells and ris of head and neck cancer 2004; 96: 449-55.
    13. Negri L, Pacchioni D, Calabrese F, Giacomasso S, MastromatteoV, Fazio M. Serum and salivary CEA and GICA levels in oral cavity tumors. Int J Biol Markers 1988; 3: 107-12. Go to original source...
    14. Mizukawa N, Sugiyama K, Fukunaga J, et al. Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma pacients. Anticancer Res 1998; 18: 4645-9.
    15. Hu S, Xie Y, Ramachandran P. Large- scale identification of proteins in human salivary proteome by liquid chromatography/mass spectrometry two-dimensional gel electrophoresis-mass spectrometry. Proteomics 2005 5 (6) 1714-1728. Go to original source... Go to PubMed...
    16. St John M, Li Y, Zhou X et al. IL 6 and IL8 potential biomarkers for oral cavity and oropharyngeal SCCA. Arch. Otolaryngol. Head Neck surg. 2004; 130: 929-935. Go to original source... Go to PubMed...
    17. Streckfus CF, Bigler L, Dellinger TD, Dai X, Kingman A, Thigpen JT. The presence of c-erbB-2, and CA 15-3 in saliva and serum among women with breast carcinoma: a preliminary study. Clin Cancer Res 2000; 6: 2363-2370.
    18. Di-Xia C, Schwartz P, Fan-Qin L Salivary and serum CA 125 assays for detecting malignant ovarian tumors. Obstet Gynecol 1990; 75: 701-704. Go to PubMed...
    19. Wright DG, Meierovics AI, Foxley JM. Assessing the delivery of neutrophils to tissues in neutropenia. Blood 1986; 67: 1023-1030. Go to original source...
    20. Gorgun A, Knight DR, Wright GD. Use of oral mucosal neutrophil counts to detect the onset and resolution of profound neutropenia following high-dose myelosuppressive chemotherapy. American Journal of Hematology 2003; 72: 13-19. Go to original source... Go to PubMed...
    21. Cheretakise C, Dror Y, Glogauer M. A non invasive oral rinse assay to monitor engraftment, neutrophil tissue delivery and susceptibility to infection following HSCI in pediatric patients. Bone Marrow Transplantation 2005; 36: 227-232. Go to original source... Go to PubMed...
    22. Lieschke GJ, Ramenghi U, OConnor MP, Sheridan W, Szer J, Morstyn G. Studies of oral neutrophils levels in patients receiving G-CSF after autologous marrow transplantation. British Journal of Haematology 1992; 82: 589-595. Go to original source... Go to PubMed...
    23. Gordis EB et. al. Asymmetry between salivary cortisol and alphaamylase reactivity to stress: relation to aggresive behavior in adolescents. Psychoneu-roendocrinology 2006; 31: 976-987. Go to original source... Go to PubMed...
    24. Chatterton RT et. al. Salivary alpha-amylase as a measure of endogenous adrenergic activity 1996; 16: 433-448. Go to original source...
    25. Bosch JA et. al. Innate secretory immunity in response to laboratory stressors that evoke distinct patterns of cardiac autonomic activity. Psychosom. Med. 2003; 65: 245-58. Go to original source... Go to PubMed...
    26. Beckett AH, Rowland M. Urinary excretion kinetics of amphetamine in man. J.Pharm. Pharmacol., 1965; 17: 628-638. Go to original source... Go to PubMed...
    27. Wan SH, Matin SB, Azarnoff DL. Kinetics, salivary excretion of amphetamine isomers, and effect of urinary pH. Clin.Pharmacol. Ther. 1978; 23: 585-590. Go to original source... Go to PubMed...
    28. Inaba T, Kalow W. Salivary excretion of amobarbital in man. Clin. Pharmacol.Ther. 1975; 18: 558-562. Go to original source... Go to PubMed...
    29. Di-Gregorio GJ, Piraino AJ, Ruch E. Diazepam concetrations in parotid saliva, mixed saliva and plasma. Clin.Pharmacol.Ther. 1978; 24: 720-725. Go to original source... Go to PubMed...
    30. Huestis MA, Cone EJ. Relationship of delta-9-tetrahydrocannabinol in oral fluid to plasma after controlled administration of smoked cannabis.J.Anal.Toxicol.2004; 28: 394-399. Go to original source... Go to PubMed...
    31. Niedbala RS, Kardos KW, Salamone S. et al. Passive cannabis smoke exposure and oral fluid testing, J.Anal.Toxicol., 2004; 28: 546-552. Go to original source... Go to PubMed...
    32. Niedbala RS, Kardos KW, Fritch EF et al. Passive cannabis smoke exposure and oral fluid testing. II. Two studies of extreme cannabis smoke exposure in a motor vehicle J.Anal.Toxicol. 2005; 29: 607- 615. Go to original source... Go to PubMed...
    33. Cone EJ, Oyler J, Darwin WD. Cocaine disposition in saliva following intravenous, intranasal, and smoked administration. J. Anal. Toxicol. 1997; 21: 465-475. Go to original source... Go to PubMed...
    34. Etzel RA. A review OF the use OF saliva conitine as a marker OF tobacco smoke exposure. Prev.Med.1990; 19: 190-197. Go to original source... Go to PubMed...
    35. Rook EJ, Van Ree JM, Van Den BW et al. Pharmacokinetics and pharmacodynamics of high doses of pharmaceutically prepared heroin, by intravenous or by inhalation route in opioid-dependent patients. Basic.Clin.Pharmacol. Toxicol. 2006; 98: 86-96. Go to original source... Go to PubMed...
    36. Jenkins AJ, Oyler JM, Cone EJ. Comparison of heroin and cocaine concetrations in saliva with concentrations in blood and plasma. J.Anal.Toxicol. 1995; 19: 359-374. Go to original source... Go to PubMed...
    37. Cone EJ, Dickerson S, Paul BD, Mitchell JM, Forensic drug testing for opiates V.Urine testing for heroin, morphine, and codeine, with commercial opiate immunoassays. J.Anal.Toxicol. 1993; 17: 156-164. Go to original source... Go to PubMed...
    38. Cone EJ. Saliva testing for drug abuse. Ann.N.Y.Acad.Sci. 1993; 694: 91-127. Go to original source... Go to PubMed...
    39. Jenkins AJ, Oyler JM, Cone EJ. Comparison of heroin and cocain concentrations in saliva with concentration in blood and plasma J.Anal.Toxicol, 1995; 19: 359-374. Go to original source... Go to PubMed...
    40. Rohrig TP, Moore C. The determination of morphine in urine and oral fluid following ingestion of poppy seeds J.Anal.Toxikol. 2003; 27: 449-452. Go to original source... Go to PubMed...
    41. Kim I, Barnes AJ, Oyler JM et al. Plasma and oral fluid pharmacokinetics and pharmacodynamics after oral codeine administration Clin.Chem. 2000; 48: 1486-1496. Go to original source...
    42. Eguchi J, Isihara K, Watanabe A, Fukumoto Y, Okuda K. PCR method is essential for detecting Mycobacterium tuberculosis in oral cavity samples. Oral.Microbiol.Immunol. 2003; 18(3): 156-159. Go to original source... Go to PubMed...
    43. Shames B, Krajden S, Fuksa M, Babida C, Penner JL. Evidence for the occurence of the same strain of Campylobacter pylori in the stomach and dental plaque. J.Clin.Microbiol., 1989; 27(12): 2849-2850. Go to original source... Go to PubMed...
    44. Tiwari SK, Khan AA, Ahmed KS et al. Rapid diagnosis of Helicobacter pylori infection in dyspeptic patients using salivary secretion: a non-invasive approach. Singapore Med.J. 2005; 46(5): 224-228. Go to PubMed...
    45. Granstrom G, Askelof P, Granstrom M. Specific immunoglobulin A to Bordetella pertussis antigens in mucosal secretion for rapid diagnosis of whooping cough. J.Clin.Microbiol., 1988; 26(5): 869- 874. Go to original source... Go to PubMed...
    46. Scully C. HIV topic update: salivary testing for antibodies, Oral. Dis.,1997; 3: 212-215. Go to original source... Go to PubMed...
    47. Martinez PM, Torres AR, Ortiz de Lejarazu R, Montoya A, Martin JF, Eiros JM. HIV antibody testing by enzyme-linked fluorescent and western blot assays using serum, gingival crevicular transudate, and urine samples. J.Clin.Microbiol. 1999; 37: 1100-1106. Go to original source...
    48. Grant RM, Piwowar EM, Katongole-Mbidde E et al. Com parison of saliva and serum for human immunodeficiency virus type 1 antibody testing in Uganda using a rapid recombinant assay. Clin. Diag.Laboratory Immunol. 1996; 3: 640-644. Go to original source... Go to PubMed...
    49. Luizzi G, Chirianni A, Clementi M, Zaccarelli M, Antinori A, Piazza M. Reduction of. HIV-1 viral load in saliva by indinavir containing antiretroviral regimen. 2002; 16(3): 503-504. Go to original source...
    50. Baron S, Poast J, Cloyd MW. Why is HIV rarely transmitted by oral secretions? Saliva can disrupt orally shed, infected leukocytes. Arch.Intern.Med., 1999; 159 (3): 303-310. Go to original source... Go to PubMed...
    51. Ly TD, Ebel A, Faucher V, Fihman V, Laperche S. Could the new HIV combined p24 antigen and antibody assays replace p24 antigen specific assays? J.Virol. Methods 2007; 143(1): 86-94. Go to original source... Go to PubMed...
    52. Blackbourn DJ, Lennette ET, Ambroziak J, Mourich DV, Levy JA. Human herpesvirus 8 detection in nasal secretion and saliva J.Infect. Dis. 1998; 177: 213-216. Go to original source... Go to PubMed...
    53. LaDuca JR, Love JL, Abott LZ, Dube S, Freidman-Kien AE, Poiesz BJ, Detestion OF human herpes virus 8 DNA sequences in tissues and bodily fluids. J.Infect. Dis. 1998; 178: 1610-1615. Go to original source... Go to PubMed...
    54. Crepin P, Audry L, Rotivel Y, Gacoin A, Caroff C, Bourhy H. Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid. J.Clin.Microbiol. 1998; 36: 1117-1121. Go to original source... Go to PubMed...
    55. Crowcroft NS, Vyse A, Brown DW, Strachan DP. Epide miology of Eppstein-Barr virus infection in pre-adolescent children: application of a new salivary method in Edinburgh, Scotland. J.Epidemiol. Community Health, 1998; 52: 101-104. Go to original source...

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