The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
ANANTIN - A PEPTIDE ANTAGONIST OF THE ATRIAL NATRIURETIC FACTOR (ANF)
I. PRODUCING ORGANISM, FERMENTATION, ISOLATION AND BIOLOGICAL ACTIVITY
W. WEBERW. FISCHLIE. HOCHULIE. KUPFERE. K. WEIBEL
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JOURNAL FREE ACCESS

1991 Volume 44 Issue 2 Pages 164-171

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Abstract

Anantin, a peptide binding to the receptor of the atrial natriuretic factor (ANF) was isolated from a strain of Streptomyces coerulescens. The molecule consists of 17 natural L-amino acids which form a peptidic ring system. It has a MW of 1, 871.0. The chemical composition is C90H111 N21O24.
The compound was found to bind competitively to ANF-receptors from bovine adrenal cortex (Kd = 0.61 μM). Furthermore, it dose-dependently inhibited the ANF-induced intracellular cyclic guanosine monophosphate accumulation in bovine aorta smooth muscle cells. At the same concentration no agonistic effects were detectable in these cells. Thus, anantin is considered to be the first microbially produced antagonist of the cardiac hormone, ANF.

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© Japan Antibiotics Research Association
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